TERRA Transcripts Are Bound by a Complex Array of RNA-binding Proteins

Overview

Journal Nat Commun

Specialty Biology

Date 2010 Oct 27

PMID 20975687

Citations 73

Authors

Isabel Lopez de Silanes

Martina Stagno dAlcontres

Maria A Blasco

Affiliations

Soon will be listed here.

Abstract

Telomeres are transcribed from the telomeric C-rich strand, giving rise to UUAGGG repeat-containing telomeric transcripts or TERRA, which are novel structural components of telomeres. TERRA abundance is highly dependent on developmental status (including nuclear reprogramming), telomere length, cellular stresses, tumour stage and chromatin structure. However, the molecular mechanisms and factors controlling TERRA levels are still largely unknown. In this study, we identify a set of RNA-binding proteins, which endogenously bind and regulate TERRA in the context of primary mouse embryonic fibroblasts. The identification was carried out by biotin pull-down assays followed by LC-MALDI TOF/TOF mass spectrometry. Different members of the heterogeneous nuclear ribonucleoprotein family are among the ribonucleoprotein family that bind more abundantly to TERRA. Downregulation of TERRA-bound RBPs by small interfering RNA further shows that they can impact on TERRA abundance, their location and telomere lengthening. These findings anticipate an impact of TERRA-associated RBPs on telomere biology and telomeres diseases, such as cancer and aging.

Citing Articles

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TERRA transcripts localize at long telomeres to regulate telomerase access to chromosome ends.

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