Identification and Expression Analysis of ERF Transcription Factor Genes in Petunia During Flower Senescence and in Response to Hormone Treatments

Overview

Journal J Exp Bot

Publisher Oxford University Press

Specialty Biology

Date 2010 Oct 27

PMID 20974735

Citations 45

Authors

Juanxu Liu

Jingyu Li

Huinan Wang

Zhaodi Fu

Juan Liu

Yixun Yu

Affiliations

Soon will be listed here.

Abstract

Ethylene-responsive element-binding factor (ERF) genes constitute one of the largest transcription factor gene families in plants. In Arabidopsis and rice, only a few ERF genes have been characterized so far. Flower senescence is associated with increased ethylene production in many flowers. However, the characterization of ERF genes in flower senescence has not been reported. In this study, 13 ERF cDNAs were cloned from petunia. Based on the sequence characterization, these PhERFs could be classified into four of the 12 known ERF families. Their predicted amino acid sequences exhibited similarities to ERFs from other plant species. Expression analyses of PhERF mRNAs were performed in corollas and gynoecia of petunia flower. The 13 PhERF genes displayed differential expression patterns and levels during natural flower senescence. Exogenous ethylene accelerates the transcription of the various PhERF genes, and silver thiosulphate (STS) decreased the transcription of several PhERF genes in corollas and gynoecia. PhERF genes of group VII showed a strong association with the rise in ethylene production in both petals and gynoecia, and might be associated particularly with flower senescence in petunia. The effect of sugar, methyl jasmonate, and the plant hormones abscisic acid, salicylic acid, and 6-benzyladenine in regulating the different PhERF transcripts was investigated. Functional nuclear localization signal analyses of two PhERF proteins (PhERF2 and PhERF3) were carried out using fluorescence microscopy. These results supported a role for petunia PhERF genes in transcriptional regulation of petunia flower senescence processes.

Citing Articles

Comparative Analysis of Floral Transcriptomes in (Malvaceae).

Nobles A, Wendel J, Yoo M Plants (Basel). 2025; 14(4).

PMID: 40006762 PMC: 11859044. DOI: 10.3390/plants14040502.

Functional Divergence of the Closely Related Genes PhARF5 and PhARF19a in Flower Formation and Hormone Signaling.

Ding Y, Miao Y, Huang L, Zhu H, Li W, Zou W Int J Mol Sci. 2024; 25(22).

PMID: 39596314 PMC: 11594976. DOI: 10.3390/ijms252212249.

Genetic Evidence of in Leaf Morphology Variation of 'Mojo'.

Yang S, Hu M, Wu R, Hou Z, Zhang H, He W Genes (Basel). 2024; 15(9).

PMID: 39336723 PMC: 11431335. DOI: 10.3390/genes15091132.

PfERF106, a novel key transcription factor regulating the biosynthesis of floral terpenoids in Primula forbesii Franch.

Yin X, Yang H, Ding K, Luo Y, Deng W, Liao J BMC Plant Biol. 2024; 24(1):851.

PMID: 39256664 PMC: 11385529. DOI: 10.1186/s12870-024-05567-7.

Analysis of the Aging-Related AP2/ERF Transcription Factor Gene Family in .

Chen G, Shao T, Zhou Y, Chen F, Zhang D, Gu H Int J Mol Sci. 2024; 25(15).

PMID: 39125596 PMC: 11312093. DOI: 10.3390/ijms25158025.

References

Zhou J, Tang X, Martin G . The Pto kinase conferring resistance to tomato bacterial speck disease interacts with proteins that bind a cis-element of pathogenesis-related genes. EMBO J. 1997; 16(11):3207-18. PMC: 1169938. DOI: 10.1093/emboj/16.11.3207. View

Sakuma Y, Liu Q, Dubouzet J, Abe H, Shinozaki K, Yamaguchi-Shinozaki K . DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration- and cold-inducible gene expression. Biochem Biophys Res Commun. 2002; 290(3):998-1009. DOI: 10.1006/bbrc.2001.6299. View

Gu Y, Yang C, Thara V, Zhou J, Martin G . Pti4 is induced by ethylene and salicylic acid, and its product is phosphorylated by the Pto kinase. Plant Cell. 2000; 12(5):771-86. PMC: 139926. DOI: 10.1105/tpc.12.5.771. View

Sakuma Y, Maruyama K, Qin F, Osakabe Y, Shinozaki K, Yamaguchi-Shinozaki K . Dual function of an Arabidopsis transcription factor DREB2A in water-stress-responsive and heat-stress-responsive gene expression. Proc Natl Acad Sci U S A. 2006; 103(49):18822-7. PMC: 1693746. DOI: 10.1073/pnas.0605639103. View

Srivastava , Dwivedi . Delayed ripening of banana fruit by salicylic acid. Plant Sci. 2000; 158(1-2):87-96. DOI: 10.1016/s0168-9452(00)00304-6. View

Qin F, Sakuma Y, Tran L, Maruyama K, Kidokoro S, Fujita Y . Arabidopsis DREB2A-interacting proteins function as RING E3 ligases and negatively regulate plant drought stress-responsive gene expression. Plant Cell. 2008; 20(6):1693-707. PMC: 2483357. DOI: 10.1105/tpc.107.057380. View

Iordachescu M, Verlinden S . Transcriptional regulation of three EIN3-like genes of carnation (Dianthus caryophyllus L. cv. Improved White Sim) during flower development and upon wounding, pollination, and ethylene exposure. J Exp Bot. 2005; 56(418):2011-8. DOI: 10.1093/jxb/eri199. View

Yang Z, Tian L, Latoszek-Green M, Brown D, Wu K . Arabidopsis ERF4 is a transcriptional repressor capable of modulating ethylene and abscisic acid responses. Plant Mol Biol. 2005; 58(4):585-96. DOI: 10.1007/s11103-005-7294-5. View

Jones M, Larsen P, Woodson W . Ethylene-regulated expression of a carnation cysteine proteinase during flower petal senescence. Plant Mol Biol. 1995; 28(3):505-12. DOI: 10.1007/BF00020397. View

10.

Onate-Sanchez L, Anderson J, Young J, Singh K . AtERF14, a member of the ERF family of transcription factors, plays a nonredundant role in plant defense. Plant Physiol. 2006; 143(1):400-9. PMC: 1761963. DOI: 10.1104/pp.106.086637. View

11.

Park J, Park C, Lee S, Ham B, Shin R, Paek K . Overexpression of the tobacco Tsi1 gene encoding an EREBP/AP2-type transcription factor enhances resistance against pathogen attack and osmotic stress in tobacco. Plant Cell. 2001; 13(5):1035-46. PMC: 135557. DOI: 10.1105/tpc.13.5.1035. View

12.

Champion A, Hebrard E, Parra B, Bournaud C, Marmey P, Tranchant C . Molecular diversity and gene expression of cotton ERF transcription factors reveal that group IXa members are responsive to jasmonate, ethylene and Xanthomonas. Mol Plant Pathol. 2009; 10(4):471-85. PMC: 6640365. DOI: 10.1111/j.1364-3703.2009.00549.x. View

13.

Yin X, Allan A, Chen K, Ferguson I . Kiwifruit EIL and ERF genes involved in regulating fruit ripening. Plant Physiol. 2010; 153(3):1280-92. PMC: 2899921. DOI: 10.1104/pp.110.157081. View

14.

Nakano T, Suzuki K, Fujimura T, Shinshi H . Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiol. 2006; 140(2):411-32. PMC: 1361313. DOI: 10.1104/pp.105.073783. View

15.

Hunter D, Ferrante A, Vernieri P, Reid M . Role of abscisic acid in perianth senescence of daffodil (Narcissus pseudonarcissus"Dutch Master"). Physiol Plant. 2004; 121(2):313-321. DOI: 10.1111/j.0031-9317.2004.0311.x. View

16.

Kumar S, Tamura K, Nei M . MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform. 2004; 5(2):150-63. DOI: 10.1093/bib/5.2.150. View

17.

Yang Y, Wu Y, Pirrello J, Regad F, Bouzayen M, Deng W . Silencing Sl-EBF1 and Sl-EBF2 expression causes constitutive ethylene response phenotype, accelerated plant senescence, and fruit ripening in tomato. J Exp Bot. 2009; 61(3):697-708. DOI: 10.1093/jxb/erp332. View

18.

Novillo F, Alonso J, Ecker J, Salinas J . CBF2/DREB1C is a negative regulator of CBF1/DREB1B and CBF3/DREB1A expression and plays a central role in stress tolerance in Arabidopsis. Proc Natl Acad Sci U S A. 2004; 101(11):3985-90. PMC: 374356. DOI: 10.1073/pnas.0303029101. View

19.

Fujimoto S, Ohta M, Usui A, Shinshi H, Ohme-Takagi M . Arabidopsis ethylene-responsive element binding factors act as transcriptional activators or repressors of GCC box-mediated gene expression. Plant Cell. 2000; 12(3):393-404. PMC: 139839. DOI: 10.1105/tpc.12.3.393. View

20.

Ma N, Tan H, Liu X, Xue J, Li Y, Gao J . Transcriptional regulation of ethylene receptor and CTR genes involved in ethylene-induced flower opening in cut rose (Rosa hybrida) cv. Samantha. J Exp Bot. 2006; 57(11):2763-73. DOI: 10.1093/jxb/erl033. View