Transplanted Bone Marrow Stem Cells Relocate to Infarct Penumbra and Co-express Endogenous Proliferative and Immature Neuronal Markers in a Mouse Model of Ischemic Cerebral Stroke

Overview

Journal BMC Neurosci

Publisher Biomed Central

Specialty Neurology

Date 2010 Oct 27

PMID 20973978

Citations 13

Authors

Xue-Mei Zhang

Fang Du

Dan Yang

Chun-Jiang Yu

Xiang-Nan Huang

Wei Liu

Jin Fu

Affiliations

Soon will be listed here.

Abstract

Background: Several studies demonstrate that neurogenesis may be induced or activated following vascular insults, which may be important for neuronal regeneration and functional recovery. Understanding the cellular mechanism underlying stroke-associated neurogenesis is of neurobiological as well as neurological/clinical relevance. The present study attempted to explore potential homing and early development of transplanted bone marrow stem cells in mouse forebrain after focal occlusion of the middle cerebral artery, an experimental model of ischemic stroke.

Results: Bone marrow stem cells isolated from donor mice were confirmed by analysis of surface antigen profile, and were pre-labeled with a lipophilic fluorescent dye PKH26, and subsequently transfused into recipient mice with middle cerebral artery coagulation. A large number of PKH26-labeled cells were detected surrounding the infarct site, most of which colocalized with immunolabelings for the proliferating cell nuclear antigen (PCNA) and some also colocalized with the immature neuronal marker doublecortin (DCX) during 1-2 weeks after the bone marrow cells transfusion.

Conclusions: The present study shows that transplanted bone marrow cells largely relocate to the infarct penumbra in ischemic mouse cerebrum. These transplanted bone marrow cells appear to undergo a process of in situ proliferation and develop into putative cortical interneurons during the early phase of experimental vascular injury.

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