The Role of Epidermal Growth Factor in the Healing Tympanic Membrane Following Perforation in Rats

Overview

Journal J Mol Histol

Specialty Biochemistry

Date 2010 Oct 23

PMID 20967565

Citations 10

Authors

Peter L Santa Maria

Sharon L Redmond

Marcus D Atlas

Reza Ghassemifar

Affiliations

Soon will be listed here.

Abstract

Epidermal Growth Factor (EGF) has been identified as playing a critical role in the wound healing process. The objective of this study is to investigate the role that EGF plays in rat tympanic membrane (TM) wound healing using two techniques, microarray and immunohistochemistry. The tympanic membranes of rats were perforated using a sterile needle and sacrificed at time points during 2 weeks following perforation. The normalized signal intensities at the time points for EGF and associated genes are presented. The rat EGF mRNA did not change significantly between time points. Five associated proteins, including heparin-binding EGF-like growth factor were found to be differentially expressed above a two fold threshold at 12 h following perforation. EGF staining was found at low levels in the uninjured TM. Levels of EGF staining increased at 24 h in the basal keratinocyte layer, became diffusely elevated in the specimen at 36 h, before a second peak in staining of the keratinocyte layer at Day 4. The staining of EGF corresponds to its multiple roles in TM wound healing.

Citing Articles

Effect of Growth Factor-Loaded Acellular Dermal Matrix/MSCs on Regeneration of Chronic Tympanic Membrane Perforations in Rats.

Cho G, Moon C, Song A, Vijayakumar K, Ang M, Jang C J Clin Med. 2021; 10(7).

PMID: 33917576 PMC: 8038787. DOI: 10.3390/jcm10071541.

The effect of epidermal growth factor on the pseudo-healing of traumatic tympanic membrane perforations.

Lou Z Braz J Otorhinolaryngol. 2019; 87(1):53-58.

PMID: 31395492 PMC: 9422542. DOI: 10.1016/j.bjorl.2019.06.011.

A comparative study to evaluate the efficacy of EGF, FGF-2, and 0.3% (w/v) ofloxacin drops on eardrum regeneration.

Lou Z, Lou Z Medicine (Baltimore). 2017; 96(30):e7654.

PMID: 28746231 PMC: 5627857. DOI: 10.1097/MD.0000000000007654.

Functional Outcomes of Heparin-Binding Epidermal Growth Factor-Like Growth Factor for Regeneration of Chronic Tympanic Membrane Perforations in Mice.

Santa Maria P, Gottlieb P, Maria C, Kim S, Puria S, Yang Y Tissue Eng Part A. 2017; 23(9-10):436-444.

PMID: 28142401 PMC: 5444491. DOI: 10.1089/ten.TEA.2016.0395.

No systemic exposure of transtympanic heparin-binding epidermal growth factor like growth factor.

Santa Maria P, Kim S, Yang Y Drug Chem Toxicol. 2016; 39(4):451-4.

PMID: 26887920 PMC: 5580497. DOI: 10.3109/01480545.2016.1143482.

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