The Association of the Clock 3111 T/C SNP with Lipids and Lipoproteins Including Small Dense Low-density Lipoprotein: Results from the Mima Study

Overview

Journal BMC Med Genet

Publisher Biomed Central

Specialty Genetics

Date 2010 Oct 22

PMID 20961464

Citations 14

Authors

Kokoro Tsuzaki

Kazuhiko Kotani

Yoshiko Sano

Shinji Fujiwara

Kaoru Takahashi

Naoki Sakane

Affiliations

Soon will be listed here.

Abstract

Background: The clock molecule plays major roles in circadian rhythmicity and regulating lipid and glucose metabolism in peripheral organs. Disruption of the circadian rhythm can lead to cardiometabolic disorders. The existence of small dense low-density lipoprotein (sdLDL) in the circulation, an abnormality of lipid metabolism, in part associated with lifestyle, is also one of risk parameters for cardiometabolic disorders. The 3111 T/C single nucleotide polymorphism (SNP) of the Clock gene has been reported to be associated with lifestyle including morning/evening preference. We investigated whether the Clock 3111 T/C SNP may affect lipids and lipoproteins including sdLDL.

Methods: In 365 community-dwelling subjects (170 men and 195 women, mean age 63 ± 14 years), the 3111 T/C SNP was genotyped using a fluorescent allele-specific DNA primer assay system. The levels of sdLDL were measured with the electrophoretic separation of lipoproteins employing the Lipoprint system.

Results: The frequency of the Clock 3111 C allele was 0.14. The area of sdLDL did not differ between the subjects with obesity and those without. In carriers of T/T homozygotes, the area of sdLDL was significantly higher compared with carriers of the C allele (T/C or C/C) (1.7 ± 3.4 vs. 0.8 ± 1.9%; p < 0.05). A multiple regression analysis showed that the area of sdLDL was significantly and negatively correlated with the Clock 3111 T/C SNP (β = -0.114, p < 0.05), independently of age, sex, body mass index, and exercise habits.

Conclusion: Our findings indicated that the Clock 3111 T/C SNP might be associated with the existence of sdLDL.

Citing Articles

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The Association of Polymorphisms in Circadian Clock and Lipid Metabolism Genes With 2 Trimester Lipid Levels and Preterm Birth.

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CLOCK 3111T/C genetic variant influences the daily rhythm of autonomic nervous function: relevance to body weight control.

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