Anticancer Potential of Aqueous Ethanol Seed Extract of Ziziphus Mauritiana Against Cancer Cell Lines and Ehrlich Ascites Carcinoma

Overview

Journal Evid Based Complement Alternat Med

Specialty Rehabilitation Medicine

Date 2010 Oct 19

PMID 20953391

Citations 17

Authors

Tulika Mishra

Madhu Khullar

Aruna Bhatia

Affiliations

Soon will be listed here.

Abstract

Ziziphus mauritiana (Lamk.) is a fruit tree that has folkloric implications against many ailments and diseases. In the present study, anticancer potential of seed extract of Ziziphus mauritiana in vitro against different cell lines (HL-60, Molt-4, HeLa, and normal cell line HGF) by MTT assay as well as in vivo against Ehrich ascites carcinoma bearing Swiss albino mice was investigated. The extract was found to markedly inhibit the proliferation of HL-60 cells. Annexin and PI binding of treated HL-60 cells indicated apoptosis induction by extract in a dose-dependent manner. The cell cycle analysis revealed a prominent increase in sub Go population at concentration of 20 μg/ml and above. Agarose gel electrophoresis confirmed DNA fragmentation in HL-60 cells after 3 h incubation with extract. The extract also exhibited potent anticancer potential in vivo. Treatment of Ehrlich ascites carcinoma bearing Swiss albino mice with varied doses (100-800 mg/kg b.wt.) of plant extract significantly reduced tumor volume and viable tumor cell count and improved haemoglobin content, RBC count, mean survival time, tumor inhibition, and percentage life span. The enhanced antioxidant status in extract-treated animals was evident from decline in levels of lipid peroxidation and increased levels of glutathione, catalase, and superoxide dismutase.

Citing Articles

Dietary Polyphenols: Review on Chemistry/Sources, Bioavailability/Metabolism, Antioxidant Effects, and Their Role in Disease Management.

Rudrapal M, Rakshit G, Singh R, Garse S, Khan J, Chakraborty S Antioxidants (Basel). 2024; 13(4).

PMID: 38671877 PMC: 11047380. DOI: 10.3390/antiox13040429.

Standardization and Pharmacological Evaluation of Extract for Sedative and Anticonvulsant Activity in Mice and Rat.

Siddique N, Ved A, Shukla K, Nigam A Antiinflamm Antiallergy Agents Med Chem. 2024; 23(1):31-38.

PMID: 38279726 DOI: 10.2174/0118715230276586231215045816.

Performance, blood and antioxidant status in dual-purpose laying hens supplemented with aqueous extract of Christ's thorn jujube (Ziziphus spina-christi L.) leaves as phytogenic agent in subtropical conditions.

El-Kholy K, Tag El-Din H, Tawfeek F, Tufarelli V, Losacco C, Alhotan R Anim Biosci. 2024; 37(5):896-907.

PMID: 38228130 PMC: 11065718. DOI: 10.5713/ab.23.0243.

Exploring the potentials of Lam. seed kernel oil as pharmaceutical oil base: Physicochemical characterization and ketoconazole soap formulation.

Pandey J, Gaire S, Sharma K, Pun D, Gyawali A, Lamichhane G Heliyon. 2023; 9(11):e21034.

PMID: 38106666 PMC: 10722321. DOI: 10.1016/j.heliyon.2023.e21034.

Morphological characterization and interspecific variation among five species of Ziziphus genus to select superiors in Iran.

Khadivi A BMC Plant Biol. 2023; 23(1):550.

PMID: 37936089 PMC: 10631032. DOI: 10.1186/s12870-023-04566-4.

References

Rafter J . Scientific basis of biomarkers and benefits of functional foods for reduction of disease risk: cancer. Br J Nutr. 2002; 88 Suppl 2:S219-24. DOI: 10.1079/BJN2002686. View

Madhusudan S, Middleton M . The emerging role of DNA repair proteins as predictive, prognostic and therapeutic targets in cancer. Cancer Treat Rev. 2005; 31(8):603-17. DOI: 10.1016/j.ctrv.2005.09.006. View

Ellman G . Tissue sulfhydryl groups. Arch Biochem Biophys. 1959; 82(1):70-7. DOI: 10.1016/0003-9861(59)90090-6. View

Mishra T, Bhatia A . Augmentation of expression of immunocytes' functions by seed extract of Ziziphus mauritiana (Lamk.). J Ethnopharmacol. 2009; 127(2):341-5. DOI: 10.1016/j.jep.2009.10.033. View

Del Bino G, Darzynkiewicz Z, Degraef C, Mosselmans R, Fokan D, Galand P . Comparison of methods based on annexin-V binding, DNA content or TUNEL for evaluating cell death in HL-60 and adherent MCF-7 cells. Cell Prolif. 1999; 32(1):25-37. PMC: 6726319. View

Ohkawa H, Ohishi N, Yagi K . Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95(2):351-8. DOI: 10.1016/0003-2697(79)90738-3. View

Fulda S, Debatin K . Betulinic acid induces apoptosis through a direct effect on mitochondria in neuroectodermal tumors. Med Pediatr Oncol. 2000; 35(6):616-8. DOI: 10.1002/1096-911x(20001201)35:6<616::aid-mpo27>3.0.co;2-n. View

Shashi B, Jaswant S, Madhusudana R, Kumar S, Nabi Q . A novel lignan composition from Cedrus deodara induces apoptosis and early nitric oxide generation in human leukemia Molt-4 and HL-60 cells. Nitric Oxide. 2005; 14(1):72-88. DOI: 10.1016/j.niox.2005.09.009. View

Marklund S, Westman N, Lundgren E, Roos G . Copper- and zinc-containing superoxide dismutase, manganese-containing superoxide dismutase, catalase, and glutathione peroxidase in normal and neoplastic human cell lines and normal human tissues. Cancer Res. 1982; 42(5):1955-61. View

10.

Vermes I, Haanen C, Steffens-Nakken H, Reutelingsperger C . A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J Immunol Methods. 1995; 184(1):39-51. DOI: 10.1016/0022-1759(95)00072-i. View

11.

Nakatani N, Ichimaru M, Moriyasu M, Kato A . Induction of apoptosis in human promyelocytic leukemia cell line HL-60 by C-benzylated dihydrochalcones, uvaretin, isouvaretin and diuvaretin. Biol Pharm Bull. 2005; 28(1):83-6. DOI: 10.1248/bpb.28.83. View

12.

Kang K, Lee H, Kim C, Lee S, Tunsag J, Batsuren D . The chemopreventive effects of Saussurea salicifolia through induction of apoptosis and phase II detoxification enzyme. Biol Pharm Bull. 2007; 30(12):2352-9. DOI: 10.1248/bpb.30.2352. View

13.

Sun Y, Oberley L, Elwell J . Antioxidant enzyme activities in normal and transformed mouse liver cells. Int J Cancer. 1989; 44(6):1028-33. DOI: 10.1002/ijc.2910440615. View

14.

Kawamura M, Kasai H . Delayed Cell Cycle Progression and Apoptosis Induced by Hemicellulase-Treated Agaricus blazei. Evid Based Complement Alternat Med. 2007; 4(1):83-94. PMC: 1810359. DOI: 10.1093/ecam/nel059. View

15.

Uddin S, Grice I, Tiralongo E . Cytotoxic effects of bangladeshi medicinal plant extracts. Evid Based Complement Alternat Med. 2009; 2011:578092. PMC: 3144735. DOI: 10.1093/ecam/nep111. View

16.

Denicourt C, Dowdy S . Medicine. Targeting apoptotic pathways in cancer cells. Science. 2004; 305(5689):1411-3. DOI: 10.1126/science.1102974. View

17.

Malik F, Kumar A, Bhushan S, Khan S, Bhatia A, Avtar Suri K . Reactive oxygen species generation and mitochondrial dysfunction in the apoptotic cell death of human myeloid leukemia HL-60 cells by a dietary compound withaferin A with concomitant protection by N-acetyl cysteine. Apoptosis. 2007; 12(11):2115-33. DOI: 10.1007/s10495-007-0129-x. View

18.

Lien H, Lin H, Wang Y, Chen L, Yang D, Lai Y . Inhibition of Anchorage-Independent Proliferation and G0/G1 Cell-Cycle Regulation in Human Colorectal Carcinoma Cells by 4,7-Dimethoxy-5-Methyl-l,3-Benzodioxole Isolated from the Fruiting Body of Antrodia camphorate. Evid Based Complement Alternat Med. 2009; 2011:984027. PMC: 3137876. DOI: 10.1093/ecam/nep020. View

19.

Gonzales G, Valerio Jr L . Medicinal plants from Peru: a review of plants as potential agents against cancer. Anticancer Agents Med Chem. 2006; 6(5):429-44. DOI: 10.2174/187152006778226486. View

20.

Bhatia A, Mishra T . Hypoglycemic activity of Ziziphus mauritiana aqueous ethanol seed extract in alloxan-induced diabetic mice. Pharm Biol. 2010; 48(6):604-10. DOI: 10.3109/13880200903218935. View