Analysis of RNA Folding by Native Polyacrylamide Gel Electrophoresis

Overview

Journal Methods Enzymol

Specialty Biochemistry

Date 2010 Oct 16

PMID 20946790

Citations 23

Authors

Sarah A Woodson

Eda Koculi

Affiliations

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Abstract

Polyacrylamide gel electrophoresis under native conditions (native PAGE) is a well-established and versatile method for probing nucleic acid conformation and nucleic acid-protein interactions. Native PAGE has been used to measure RNA folding equilibria and kinetics under a wide variety of conditions. Advantages of this method are its adaptability, absolute determination of reaction endpoints, and direct analysis of conformational hetereogeneity within a sample. Native PAGE is also useful for resolving ligand-induced structural changes.

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