Anti-inflammatory Defense Mechanisms of Entamoeba Histolytica

Overview

Journal Inflamm Res

Specialties Allergy & Immunology
Pathology

Date 2010 Oct 13

PMID 20938711

Citations 7

Authors

Raul Silva-Garcia

Guadalupe Rico-Rosillo

Affiliations

Soon will be listed here.

Abstract

The monocyte locomotion inhibitory factor (MLIF), a heat-stable oligopeptide found in the supernatant fluid of Entamoeba histolytica axenic cultures, may contribute to the delayed inflammation observed in amoebic hepatic abscess. This factor was isolated by ultra-filtration and high powered liquid chromatography, obtaining a primary Met-Gln-Cys-Asn-Ser structure, identified afterwards as the carboxyl-terminal (…Cys-Asn-Ser) active site. The selective anti-inflammatory effects of the pentapeptide have been observed in both in vitro and in vivo models, using a synthetic pentapeptide to maintain the same anti-inflammatory conditions during the experimental assays. Anti-inflammatory effects observed include inhibition of human monocyte locomotion and the respiratory burst in monocytes and neutrophils, increasing expression of anti-inflammatory cytokines and inhibiting expression of the adhesion molecules VLA-4 and VCAM, among others. In this review, we will describe the effects of MLIF detected so far and how it might be used as a therapeutical agent against inflammatory diseases.

Citing Articles

Bioactive peptides and proteins for tissue repair: microenvironment modulation, rational delivery, and clinical potential.

Hao Z, Zhang Z, Wang Z, Wang Y, Chen J, Chen T Mil Med Res. 2024; 11(1):75.

PMID: 39639374 PMC: 11619216. DOI: 10.1186/s40779-024-00576-x.

Use of Cells, Supplements, and Peptides as Therapeutic Strategies for Modulating Inflammation after Spinal Cord Injury: An Update.

Garcia E, Buzoianu-Anguiano V, Silva-Garcia R, Esparza-Salazar F, Arriero-Cabanero A, Escandon A Int J Mol Sci. 2023; 24(18).

PMID: 37762251 PMC: 10531377. DOI: 10.3390/ijms241813946.

Anti-Inflammatory Dipeptide, a Metabolite from Ambioba Secretion, Protects Cerebral Ischemia Injury by Blocking Apoptosis Via p-JNK/Bax Pathway.

Zhang Q, Dai J, Song Z, Guo Y, Deng S, Yu Y Front Pharmacol. 2021; 12:689007.

PMID: 34220513 PMC: 8249563. DOI: 10.3389/fphar.2021.689007.

New monocyte locomotion inhibitory factor analogs protect against cerebral ischemia-reperfusion injury in rats.

Wang X, Wang C, Yang Y, Ni J Bosn J Basic Med Sci. 2017; 17(3):221-227.

PMID: 28273031 PMC: 5581970. DOI: 10.17305/bjbms.2017.1622.

EF1A1/HSC70 Cooperatively Suppress Brain Endothelial Cell Apoptosis via Regulating JNK Activity.

Liu Y, Jiang S, Yang P, Zhang Y, Li T, Rui Y CNS Neurosci Ther. 2016; 22(10):836-44.

PMID: 27324700 PMC: 6492866. DOI: 10.1111/cns.12581.

References

Silva Garcia R, Rico Rosillo G, Espinosa Cantellano M, Castanon G, Gimenez Scherer J, Kretschmer R . Entamoeba dispar does not produce the monocyte locomotion inhibitory factor (MLIF) produced by Entamoeba histolytica. Parasite Immunol. 2003; 25(2):99-101. DOI: 10.1046/j.1365-3024.2003.00608.x. View

Lien S, Lowman H . Therapeutic peptides. Trends Biotechnol. 2003; 21(12):556-62. DOI: 10.1016/j.tibtech.2003.10.005. View

Jenssen H, Hamill P, Hancock R . Peptide antimicrobial agents. Clin Microbiol Rev. 2006; 19(3):491-511. PMC: 1539102. DOI: 10.1128/CMR.00056-05. View

Ghosh T, Gupta S, Majumdar S . Studies on codon usage in Entamoeba histolytica. Int J Parasitol. 2000; 30(6):715-22. DOI: 10.1016/s0020-7519(00)00042-4. View

Kretschmer R, Salinas-Carmona M, Estela Avila M . [Effect of Entamoeba histolytica on the chemotaxis of human monocytes]. Arch Invest Med (Mex). 1980; 11(1 Suppl):147-51. View

Viveros-Rogel M, Salvatierra B, Tapia-Conyer R, Sepulveda-Amor J, Gutierrez G, Ortiz-Ortiz L . Seroepidemiology of amebiasis in Mexico. Am J Trop Med Hyg. 1994; 50(4):412-9. DOI: 10.4269/ajtmh.1994.50.412. View

Wolpe S, Davatelis G, Sherry B, Beutler B, Hesse D, Nguyen H . Macrophages secrete a novel heparin-binding protein with inflammatory and neutrophil chemokinetic properties. J Exp Med. 1988; 167(2):570-81. PMC: 2188834. DOI: 10.1084/jem.167.2.570. View

Bilezikjian L, Blount A, Donaldson C, Vale W . Pituitary actions of ligands of the TGF-beta family: activins and inhibins. Reproduction. 2006; 132(2):207-15. DOI: 10.1530/rep.1.01073. View

Rico G, Leandro E, Rojas S, Gimenez J, Kretschmer R . The monocyte locomotion inhibitory factor produced by Entamoeba histolytica inhibits induced nitric oxide production in human leukocytes. Parasitol Res. 2003; 90(4):264-7. DOI: 10.1007/s00436-002-0780-7. View

10.

Gimenez Scherer J, Rico G, Kretschmer R . Inhibition of contact cutaneous delayed hypersensitivity reactions to DNBC in guinea pigs by the monocyte locomotion inhibitory factor (MLIF) produced by axenically grown Entamoeba histolytica. Arch Med Res. 1997; 28 Spec No:237-8. View

11.

Gharaee-Kermani M, Phan S . Role of cytokines and cytokine therapy in wound healing and fibrotic diseases. Curr Pharm Des. 2001; 7(11):1083-103. DOI: 10.2174/1381612013397573. View

12.

Rico G, Gimenez-Scherer J, Kretschmer R . Effect of the monocyte locomotion inhibitory factor (MLIF) produced by Entamoeba histolytica upon the respiratory burst of human leukocytes. Arch Med Res. 1992; 23(2):157-9. View

13.

Al-Shahrour F, Minguez P, Vaquerizas J, Conde L, Dopazo J . BABELOMICS: a suite of web tools for functional annotation and analysis of groups of genes in high-throughput experiments. Nucleic Acids Res. 2005; 33(Web Server issue):W460-4. PMC: 1160217. DOI: 10.1093/nar/gki456. View

14.

Balemans W, Van Hul W . Extracellular regulation of BMP signaling in vertebrates: a cocktail of modulators. Dev Biol. 2002; 250(2):231-50. View

15.

Kozian D, Ziche M, Augustin H . The activin-binding protein follistatin regulates autocrine endothelial cell activity and induces angiogenesis. Lab Invest. 1997; 76(2):267-76. View

16.

Kennedy K, Karpus W . Role of chemokines in the regulation of Th1/Th2 and autoimmune encephalomyelitis. J Clin Immunol. 1999; 19(5):273-9. DOI: 10.1023/a:1020535423465. View

17.

Busold C, Winter S, Hauser N, Bauer A, Dippon J, Hoheisel J . Integration of GO annotations in Correspondence Analysis: facilitating the interpretation of microarray data. Bioinformatics. 2005; 21(10):2424-9. DOI: 10.1093/bioinformatics/bti367. View

18.

Tannich E, Horstmann R . Codon usage in pathogenic Entamoeba histolytica. J Mol Evol. 1992; 34(3):272-3. DOI: 10.1007/BF00162976. View

19.

Gimenez-Scherer J, Cruz de Lavin E, Hernandez-Jauregui P, Merchant M, Kretschmer R . Ultrastructural changes associated with the inhibition of monocyte chemotaxis caused by products of axenically grown Entamoeba histolytica. Lab Invest. 1987; 57(1):45-51. View

20.

Kretschmer R, Rico G, Gimenez J . A novel anti-inflammatory oligopeptide produced by Entamoeba histolytica. Mol Biochem Parasitol. 2001; 112(2):201-9. DOI: 10.1016/s0166-6851(00)00367-4. View