A Self-powered, One-step Chip for Rapid, Quantitative and Multiplexed Detection of Proteins from Pinpricks of Whole Blood

Overview

Journal Lab Chip

Publisher Royal Society of Chemistry

Specialties Biotechnology
Chemistry

Date 2010 Oct 7

PMID 20924527

Citations 31

Authors

Jun Wang

Habib Ahmad

Chao Ma

Qihui Shi

Ophir Vermesh

Udi Vermesh

James Heath

Affiliations

Soon will be listed here.

Abstract

We describe an automated, self-powered chip based on lateral flow immunoassay for rapid, quantitative, and multiplex protein detection from pinpricks of whole blood. The device incorporates on-chip purification of blood plasma by employing inertial forces to focus blood cells away from the assay surface, where plasma proteins are captured and detected on antibody "barcode" arrays. Power is supplied from the capillary action of a piece of adsorbent paper, and sequentially drives, over a 40 minute period, the four steps required to capture serum proteins and then develop a multiplex immunoassay. An 11 protein panel is assayed from whole blood, with high sensitivity and high reproducibility. This inexpensive, self-contained, and easy to operate chip provides a useful platform for point-of-care diagnoses, particularly in resource-limited settings.

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