Competitive Antagonism by Phentolamine of Responses to Biogenic Amines and the Transmitter at a Neuroglandular Junction

Overview

Journal J Physiol

Specialty Physiology

Date 1978 Jun 1

PMID 209179

Citations 9

Authors

F Bowser-Riley

C R House

R K Smith

Affiliations

Soon will be listed here.

Abstract

1. A quantitative study has been made of phentolamine's inhibition of the electrical and secretory responses of the isolated salivary glands of Nauphoeta cinerea Olivier to nerve stimulation and bath applications of agonists. 2. The results suggested that phentolamine is a competitive antagonist having an affinity constant of about 1 micrometer-1 for the receptors for dopamine, noradrenaline, adrenaline and the neurotransmitter. 3. Phentolamine also inhibited responses to 5-hydroxytryptamine in a manner seemingly more complex than competitive antagonism. Attempts to estimate the affinity constant gave values of about 0.08 and 0.015 microgram-1 for inhibition of the secretory and electrical responses respectively. 4. This investigation showed that phentolamine discriminates between two kinds of receptor in this gland, one binding 5-hydroxytryptamine and the other combining with catecholamines and the neurotransmitter.

Citing Articles

A calcium-readmission response recorded from Nauphoeta salivary gland acinar cells.

GINSBORG B, House C, Mitchell M J Physiol. 1980; 304:437-47.

PMID: 7441545 PMC: 1282940. DOI: 10.1113/jphysiol.1980.sp013334.

alpha-Flupenthixol: an antagonist of dopamine-evoked fluid secretion by an insect salivary gland preparation.

Breward J, House C, Smith R Br J Pharmacol. 1980; 69(1):123-9.

PMID: 7378651 PMC: 2044187. DOI: 10.1111/j.1476-5381.1980.tb10891.x.

A novel effect of cobalt treatment on calcium-dependent responses of the cockroach salivary gland.

Mitchell M, GINSBORG B, House C Experientia. 1980; 36(1):98-9.

PMID: 7358148 DOI: 10.1007/BF02003998.

Multiple receptor types for octopamine in the locust.

Evans P J Physiol. 1981; 318:99-122.

PMID: 6275071 PMC: 1245480. DOI: 10.1113/jphysiol.1981.sp013853.

Characterization of the dopamine receptor mediating the hyperpolarization of cockroach salivary gland acinar cells in vitro.

Evans A, Green K Br J Pharmacol. 1990; 101(1):103-8.

PMID: 2282452 PMC: 1917631. DOI: 10.1111/j.1476-5381.1990.tb12097.x.

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