Detection of DNA Fragmentation and Meiotic Segregation in Human with Isolated Teratozoospermia

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2010 Sep 28

PMID 20872065

Citations 16

Authors

Sonia Brahem

Meriem Mehdi

Hatem Elghezal

Ali Saad

Affiliations

Soon will be listed here.

Abstract

Purpose: To evaluate levels of DNA fragmentation and chromosomal abnormalities in ejaculated sperm of males with isolated teratozoospermia and to determine if specific sperm morphological types occur simultaneously with these nuclear defects.

Methods: Sperm obtained from isolated teratozoospermic men (n = 70) and fertile men (n = 30) were analysed using fluorescence in situ hybridization and TUNEL assay.

Results: Teratozoospermic men, compared to fertile men, showed significantly higher rates of sex chromosomes disomy, and diploidy. Significant correlations were found between amorphous head, microcephalic head, short tail, and sex chromosomes disomy. Level of sperm DNA fragmentation was significantly higher in teratozoospermic men than in controls and positively correlated to the incidence of macrocephalic heads, amorphous heads, and short flagella.

Conclusions: Patients with isolated teratozoospermia have increased levels of DNA fragmentation and chromosomal aneuploidy. Some specific morphological abnormalities were shown to be predictive of chromosomal abnormalities and DNA alteration.

Citing Articles

Selected Spermatozoa at Conventional Magnification Cannot Guarantee in Obtaining Spermatozoa With Long Telomere Length in Severe Teratozoospermia Patients.

Shakeri F, Nabi A, Farashahi E, Erfanian S, Agha-Rahimi A Cureus. 2025; 17(1):e77240.

PMID: 39925560 PMC: 11807394. DOI: 10.7759/cureus.77240.

Enhancing Intracytoplasmic Sperm Injection Outcomes With Zeta Sperm Selection: A Case Report.

Singh S, Nair N, Pareek C, More A, Kalbande A Cureus. 2024; 16(7):e64809.

PMID: 39156240 PMC: 11330090. DOI: 10.7759/cureus.64809.

Novel compound heterozygous mutations in cause primary infertility in Han Chinese males with multiple morphological abnormalities of the sperm flagella.

Wang M, Yang Q, Zhou J, Tan H, Hu J, Jin L Asian J Androl. 2022; 25(4):512-519.

PMID: 36510862 PMC: 10411265. DOI: 10.4103/aja202292.

Sperm DNA Fragmentation in Reproductive Medicine: A Review.

Alahmar A, Singh R, Palani A J Hum Reprod Sci. 2022; 15(3):206-218.

PMID: 36341018 PMC: 9635374. DOI: 10.4103/jhrs.jhrs_82_22.

Impaired semen quality, an increase of sperm morphological defects and DNA fragmentation associated with environmental pollution in urban population of young men from Western Siberia, Russia.

Kleshchev M, Osadchuk A, Osadchuk L PLoS One. 2021; 16(10):e0258900.

PMID: 34679097 PMC: 8535459. DOI: 10.1371/journal.pone.0258900.

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