H2A.Z Maintenance During Mitosis Reveals Nucleosome Shifting on Mitotically Silenced Genes

Overview

Journal Mol Cell

Publisher Cell Press

Specialty Cell Biology

Date 2010 Sep 25

PMID 20864037

Citations 79

Authors

Theresa K Kelly

Tina Branscombe Miranda

Gangning Liang

Benjamin P Berman

Joy C Lin

Amos Tanay

Peter A Jones

Affiliations

Soon will be listed here.

Abstract

Profound chromatin changes occur during mitosis to allow for gene silencing and chromosome segregation followed by reactivation of memorized transcription states in daughter cells. Using genome-wide sequencing, we found H2A.Z-containing +1 nucleosomes of active genes shift upstream to occupy TSSs during mitosis, significantly reducing nucleosome-depleted regions. Single-molecule analysis confirmed nucleosome shifting and demonstrated that mitotic shifting is specific to active genes that are silenced during mitosis and, thus, is not seen on promoters, which are silenced by methylation or mitotically expressed genes. Using the GRP78 promoter as a model, we found H3K4 trimethylation is also maintained while other indicators of active chromatin are lost and expression is decreased. These key changes provide a potential mechanism for rapid silencing and reactivation of genes during the cell cycle.

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