Isolation and Characterization of Equine Amniotic Fluid-derived Multipotent Stem Cells

Overview

Journal Cytotherapy

Publisher Elsevier

Specialties Cell Biology
Pharmacology

Date 2010 Sep 24

PMID 20860427

Citations 14

Authors

Sang-Bum Park

Min-Soo Seo

Jun-Gu Kang

Joon-Seok Chae

Kyung-Sun Kang

Affiliations

Soon will be listed here.

Abstract

Background Aims: Amniotic fluid (AF) is a well-known source of stem cells. However, there have been no reports regarding equine AF stem cells. We have isolated equine AF-derived multipotent stem cells (MSC) (eAF-MSC) and show that these cells exhibit self-renewal ability and multilineage differentiation.

Methods: AF was obtained from thoroughbred mares and mononuclear cells (MNC) were isolated by Ficoll-Paque density gradient. We measured the cumulative population doubling level (CPDL) and characterized the immunophenotype by flow cytometry. To investigate differentiation ability, a trilineage differentiation assay was conducted.

Results: eAF-MSC could be isolated and the proliferation level was high. eAF-MSC presented typical MSC phenotypic markers, as determined by flow cytometry. Moreover, eAF-MSC showed a trilineage differentiation capability.

Conclusions: Equine AF is a good source of MSC. Furthermore, eAF-MSC may be useful as a cell therapy application for horses.

Citing Articles

Effect of Passage Number of Conditioned Medium Collected from Equine Amniotic Fluid Mesenchymal Stem Cells: Porcine Oocyte Maturation and Embryo Development.

Park A, Oh H, Ji K, Choi E, Kim D, Kim E Int J Mol Sci. 2022; 23(12).

PMID: 35743012 PMC: 9224282. DOI: 10.3390/ijms23126569.

Effects of continuous exposure to low concentration of ClO gas on the growth, viability, and maintenance of undifferentiated MSCs in long-term cultures.

Sogawa K, Okawa R, Yachiku K, Shiozaki M, Miura T, Takayanagi H Regen Ther. 2020; 14:184-190.

PMID: 32128355 PMC: 7042415. DOI: 10.1016/j.reth.2019.12.007.

Placental Stem Cells from Domestic Animals: Translational Potential and Clinical Relevance.

Barboni B, Russo V, Berardinelli P, Mauro A, Valbonetti L, Sanyal H Cell Transplant. 2018; 27(1):93-116.

PMID: 29562773 PMC: 6434480. DOI: 10.1177/0963689717724797.

Villous Chorion: A Potential Source for Pluripotent-like Stromal Cells.

Kannaiyan J, Muthukutty P, Iqbal M, Paulraj B J Nat Sci Biol Med. 2017; 8(2):221-228.

PMID: 28781492 PMC: 5523533. DOI: 10.4103/0976-9668.210011.

Amniotic fluid cells: current progress and emerging challenges in renal regeneration.

Da Sacco S, Perin L, Sedrakyan S Pediatr Nephrol. 2017; 33(6):935-945.

PMID: 28620747 DOI: 10.1007/s00467-017-3711-7.