Detection of Characteristic Distributions of Phospholipid Head Groups and Fatty Acids on Neurite Surface by Time-of-flight Secondary Ion Mass Spectrometry

Overview

Journal Med Mol Morphol

Publisher Springer

Specialties Cell Biology
Molecular Biology
Pathology

Date 2010 Sep 22

PMID 20857264

Citations 13

Authors

Hyun-Jeong Yang

Itsuko Ishizaki

Noriaki Sanada

Nobuhiro Zaima

Yuki Sugiura

Ikuko Yao

Koji Ikegami

Mitsutoshi Setou

Affiliations

Soon will be listed here.

Abstract

Neurons have a large surface because of their long and thin neurites. This surface is composed of a lipid bilayer. Lipids have not been actively investigated so far because of some technical difficulties, although evidence from cell biology is emerging that lipids contain valuable information about their roles in the central nervous system. Recent progress in techniques, e.g., mass spectrometry, opens a new epoch of lipid research. We show herein the characteristic localization of phospholipid components in neurites by means of time-of-flight secondary ion mass spectrometry. We used explant cultures of mouse superior cervical ganglia, which are widely used by neurite investigation research. In a positive-ion detection mode, phospholipid head group molecules were predominantly detected. The ions of m/z 206.1 [phosphocholine, a common component of phosphatidylcholine (PC) and sphingomyelin (SM)] were evenly distributed throughout the neurites, whereas the ions of m/z 224.1, 246.1 (glycerophosphocholine, a part of PC, but not SM) showed relatively strong intensity on neurites adjacent to soma. In a negative-ion detection mode, fatty acids such as oleic and palmitic acids were mainly detected, showing high intensity on neurites adjacent to soma. Our results suggest that lipid components on the neuritic surface show characteristic distributions depending on neurite region.

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References

Dexter D, Carter C, WELLS F, Agid Y, Lees A, Jenner P . Basal lipid peroxidation in substantia nigra is increased in Parkinson's disease. J Neurochem. 1989; 52(2):381-9. DOI: 10.1111/j.1471-4159.1989.tb09133.x. View

Sugiura Y, Shimma S, Konishi Y, Yamada M, Setou M . Imaging mass spectrometry technology and application on ganglioside study; visualization of age-dependent accumulation of C20-ganglioside molecular species in the mouse hippocampus. PLoS One. 2008; 3(9):e3232. PMC: 2532745. DOI: 10.1371/journal.pone.0003232. View

Roddy T, Cannon Jr D, Meserole C, Winograd N, Ewing A . Imaging of freeze-fractured cells with in situ fluorescence and time-of-flight secondary ion mass spectrometry. Anal Chem. 2002; 74(16):4011-9. DOI: 10.1021/ac0255734. View

van Meer G, Voelker D, Feigenson G . Membrane lipids: where they are and how they behave. Nat Rev Mol Cell Biol. 2008; 9(2):112-24. PMC: 2642958. DOI: 10.1038/nrm2330. View

Sjovall P, Lausmaa J, Nygren H, Carlsson L, Malmberg P . Imaging of membrane lipids in single cells by imprint-imaging time-of-flight secondary ion mass spectrometry. Anal Chem. 2003; 75(14):3429-34. DOI: 10.1021/ac0207675. View

Fletcher J, Lockyer N, Vaidyanathan S, Vickerman J . TOF-SIMS 3D biomolecular imaging of Xenopus laevis oocytes using buckminsterfullerene (C60) primary ions. Anal Chem. 2007; 79(6):2199-206. DOI: 10.1021/ac061370u. View

Altelaar A, Klinkert I, Jalink K, de Lange R, Adan R, Heeren R . Gold-enhanced biomolecular surface imaging of cells and tissue by SIMS and MALDI mass spectrometry. Anal Chem. 2006; 78(3):734-42. DOI: 10.1021/ac0513111. View

Ikegami K, Kato S, Koike T . N-alpha-p-tosyl-L-lysine chloromethyl ketone (TLCK) suppresses neuritic degeneration caused by different experimental paradigms including in vitro Wallerian degeneration. Brain Res. 2004; 1030(1):81-93. DOI: 10.1016/j.brainres.2004.09.050. View

Volkel W, Sicilia T, Pahler A, Gsell W, Tatschner T, Jellinger K . Increased brain levels of 4-hydroxy-2-nonenal glutathione conjugates in severe Alzheimer's disease. Neurochem Int. 2006; 48(8):679-86. DOI: 10.1016/j.neuint.2005.12.003. View

10.

Monroe E, Jurchen J, Lee J, Rubakhin S, Sweedler J . Vitamin E imaging and localization in the neuronal membrane. J Am Chem Soc. 2005; 127(35):12152-3. DOI: 10.1021/ja051223y. View

11.

Roots B . Locating lipids in the CNS: an historical perspective. Neurochem Res. 1995; 20(11):1261-8. DOI: 10.1007/BF00992500. View

12.

de Chaves E, Bussiere M, MacInnis B, Vance D, Campenot R, Vance J . Ceramide inhibits axonal growth and nerve growth factor uptake without compromising the viability of sympathetic neurons. J Biol Chem. 2001; 276(39):36207-14. DOI: 10.1074/jbc.M104282200. View

13.

Kuerschner L, Ejsing C, Ekroos K, Shevchenko A, Anderson K, Thiele C . Polyene-lipids: a new tool to image lipids. Nat Methods. 2005; 2(1):39-45. DOI: 10.1038/nmeth728. View

14.

Vance J, Campenot R, Vance D . The synthesis and transport of lipids for axonal growth and nerve regeneration. Biochim Biophys Acta. 2000; 1486(1):84-96. DOI: 10.1016/s1388-1981(00)00050-0. View

15.

Taira S, Sugiura Y, Moritake S, Shimma S, Ichiyanagi Y, Setou M . Nanoparticle-assisted laser desorption/ionization based mass imaging with cellular resolution. Anal Chem. 2008; 80(12):4761-6. DOI: 10.1021/ac800081z. View

16.

Setou M, Tanaka Y, Kanai Y, Takei Y, Kawagishi M, Hirokawa N . Glutamate-receptor-interacting protein GRIP1 directly steers kinesin to dendrites. Nature. 2002; 417(6884):83-7. DOI: 10.1038/nature743. View

17.

Ikegami K, Heier R, Taruishi M, Takagi H, Mukai M, Shimma S . Loss of alpha-tubulin polyglutamylation in ROSA22 mice is associated with abnormal targeting of KIF1A and modulated synaptic function. Proc Natl Acad Sci U S A. 2007; 104(9):3213-8. PMC: 1802010. DOI: 10.1073/pnas.0611547104. View

18.

Sugiura Y, Setou M . Imaging mass spectrometry for visualization of drug and endogenous metabolite distribution: toward in situ pharmacometabolomes. J Neuroimmune Pharmacol. 2009; 5(1):31-43. DOI: 10.1007/s11481-009-9162-6. View

19.

Shimma S, Sugiura Y, Hayasaka T, Hoshikawa Y, Noda T, Setou M . MALDI-based imaging mass spectrometry revealed abnormal distribution of phospholipids in colon cancer liver metastasis. J Chromatogr B Analyt Technol Biomed Life Sci. 2007; 855(1):98-103. DOI: 10.1016/j.jchromb.2007.02.037. View

20.

Sugiura Y, Konishi Y, Zaima N, Kajihara S, Nakanishi H, Taguchi R . Visualization of the cell-selective distribution of PUFA-containing phosphatidylcholines in mouse brain by imaging mass spectrometry. J Lipid Res. 2009; 50(9):1776-88. PMC: 2724791. DOI: 10.1194/jlr.M900047-JLR200. View