Human Hedgehog Interacting Protein Expression and Promoter Methylation in Medulloblastoma Cell Lines and Primary Tumor Samples

Overview

Journal J Neurooncol

Publisher Springer

Date 2010 Sep 21

PMID 20853133

Citations 17

Authors

Mehdi H Shahi

Mohammad Afzal

Subrata Sinha

Charles G Eberhart

Juan A Rey

Xing Fan

Javier S Castresana

Affiliations

Soon will be listed here.

Abstract

Medulloblastoma is the most common pediatric brain tumor and its development is affected by genetic and epigenetic factors. In this study we found there is low or no expression of the hedgehog interacting protein (HHIP), a negative regulator of the sonic hedgehog pathway, in most medulloblastoma cell lines and primary samples explored. We proceeded to promoter methylation assays of this gene by MCA-Meth, and found that HHIP was hypermethylated in all medulloblastoma cell lines, but only in 2 out of 14 (14%) primary tumor samples. Methylation correlated with low or unexpressed HHIP in cell lines but not in primary tumor samples. These results suggest the possibility of epigenetic regulation of HHIP in medulloblastoma, similarly to gastric, hepatic and pancreatic cancer. However, HHIP seems to be not only under regulation of promoter methylation, but under other factors involved in the control of its low levels of expression in medulloblastoma.

Citing Articles

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