Morphological and Biological Characterization of Cell Line Developed from Bovine Echinococcus Granulosus

Overview

Journal In Vitro Cell Dev Biol Anim

Publisher Springer

Specialties Biology
Cell Biology

Date 2010 Sep 17

PMID 20844980

Citations 4

Authors

Claudia I Echeverria

Dora M Isolabella

Elio A Prieto Gonzalez

Araceli Leonardelli

Laura Prada

Alina Perrone

Alicia G Fuchs

Affiliations

Soon will be listed here.

Abstract

The taeniid tapeworm Echinococcus granulosus is the causative agent of echinococcal disease, a major zoonosis with worldwide distribution. Several efforts to establish an in vitro model of E. granulosus have been undertaken; however, many of them have been designed for Echinococcus multilocularis. In the present study, we have described and characterized a stable cell line obtained from E. granulosus bovine protoscoleces maintained 3 yr in vitro. Growth characterization, morphology by light, fluorescent and electronic microscopy, and karyotyping were carried out. Cell culture origin was confirmed by immunofluorescent detection of AgB4 antigen and by PCR for the mitochondrial cytochrome c-oxidase subunit 1 (DCO1) gene. Cells seeded in agarose biphasic culture resembled a cystic structure, similar to the one formed in secondary hosts. This cell line could be a useful tool to research equinococcal behavior, allowing additional physiological and pharmacological studies, such as the effect of growth factors, nutrients, and antiparasitic drugs on cell viability and growth and on cyst formation.

Citing Articles

Establishment and application of unbiased in vitro drug screening assays for the identification of compounds against Echinococcus granulosus sensu stricto.

Kaethner M, Preza M, Kaempfer T, Zumstein P, Tamponi C, Varcasia A PLoS Negl Trop Dis. 2023; 17(8):e0011343.

PMID: 37540716 PMC: 10431624. DOI: 10.1371/journal.pntd.0011343.

Characterization of the B-Cell Epitopes of Histones H4 and H2A Recognized by Sera From Patients With Liver Cysts.

Maglioco A, Aguero F, Valacco M, Valdez A, Paulino M, Fuchs A Front Cell Infect Microbiol. 2022; 12:901994.

PMID: 35770070 PMC: 9234146. DOI: 10.3389/fcimb.2022.901994.

Current Understandings of Molecular Biology of Echinococcus multilocularis, a Pathogen for Alveolar Echinococcosis in Humans- a Narrative Review Article.

Wang X, Ding J, Guo X, Zheng Y Iran J Parasitol. 2015; 10(3):329-37.

PMID: 26622288 PMC: 4662733.

Lipid-free antigen B subunits from echinococcus granulosus: oligomerization, ligand binding, and membrane interaction properties.

Silva-Alvarez V, Franchini G, Porfido J, Kennedy M, Ferreira A, Corsico B PLoS Negl Trop Dis. 2015; 9(3):e0003552.

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