Whole Sheep Ovary Cryopreservation: Evaluation of a Slow Freezing Protocol with Dimethylsulphoxide

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2010 Sep 16

PMID 20842419

Citations 5

Authors

Milan Milenkovic

Ann Wallin

Manda Ghahremani

Mats Brannstrom

Affiliations

Soon will be listed here.

Abstract

Purpose: To evaluate a slow freezing method for whole ovary cryopreservation by evaluating effects of added cryoprotectant.

Methods: Sheep ovaries were isolated during surgery, flushed with either Ringer-Acetate or dimethylsulphoxide and cryopreserved by slow freezing. After rapid thawing, viability was assessed by ovarian in vitro perfusion, cell culture, histology and fluorescent live-dead assay.

Results: Production of cyclic AMP and progesterone was slightly higher in the dimethylsulphoxide group. Cultured ovarian cells from dimethylsulphoxide-preserved ovaries secreted larger amounts of progesterone than cells from Ringer-Acetate preserved. Light microscopy of ovarian biopsies obtained after perfusion, revealed well-preserved tissue in the dimethysulphoxide group but not in the Ringer-Acetate group. The density of small follicles and ovarian cell viability were higher in dimethysulphoxide ovaries compared to Ringer-Acetate ovaries.

Conclusions: Equilibrium with its protective effect can be achieved by slow freezing protocol, with an additional protective effect by the presence of dimethylsulphoxide.

Citing Articles

Evaluation of Cryopreservation of Bovine Ovarian Tissue by Analysis of Reactive Species of Oxygen, Toxicity, Morphometry, and Morphology.

Bizarro-Silva C, Bergamo L, Costa C, Gonzalez S, Yokomizo D, Rossaneis A Vet Sci. 2024; 11(11).

PMID: 39591353 PMC: 11598973. DOI: 10.3390/vetsci11110579.

Whole Ovary Cryopreservation and Transplantation: A Systematic Review of Challenges and Research Developments in Animal Experiments and Humans.

Hossay C, Donnez J, Dolmans M J Clin Med. 2020; 9(10).

PMID: 33023111 PMC: 7601276. DOI: 10.3390/jcm9103196.

Comparison of the enzymatic efficiency of Liberase TM and tumor dissociation enzyme: effect on the viability of cells digested from fresh and cryopreserved human ovarian cortex.

Schmidt V, Isachenko V, Rappl G, Rahimi G, Hanstein B, Morgenstern B Reprod Biol Endocrinol. 2018; 16(1):57.

PMID: 29859539 PMC: 5985056. DOI: 10.1186/s12958-018-0374-6.

Cryopreservation and re-culture of a 2.3 litre biomass for use in a bioartificial liver device.

Kilbride P, Lamb S, Gibbons S, Bundy J, Erro E, Selden C PLoS One. 2017; 12(8):e0183385.

PMID: 28841674 PMC: 5572048. DOI: 10.1371/journal.pone.0183385.

The human postmenopausal ovary as a tool for evaluation of cryopreservation protocols towards whole ovary cryopreservation.

Milenkovic M, Gharemani M, Bergh A, Wallin A, Molne J, Fazlagic E J Assist Reprod Genet. 2011; 28(5):453-60.

PMID: 21350836 PMC: 3151357. DOI: 10.1007/s10815-011-9547-3.

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