Palmitic Acid Induces the Opening of a Ca2+-dependent Pore in the Plasma Membrane of Red Blood Cells: the Possible Role of the Pore in Erythrocyte Lysis

Overview

Journal J Membr Biol

Date 2010 Sep 14

PMID 20835705

Citations 5

Authors

Konstantin N Belosludtsev

Alexander S Trudovishnikov

Natalia V Belosludtseva

Alexey V Agafonov

Galina D Mironova

Affiliations

Soon will be listed here.

Abstract

Earlier we found that in the presence of Ca(2+) palmitic acid (Pal) increases the nonspecific permeability of artificial (planar and liposomal) membranes and causes permeabilization of the inner mitochondrial membrane. An assumption was made that the mechanism of Pal/Ca(2+)-induced membrane permeabilization relates to the Ca(2+)-induced phase separation of Pal and can be considered as formation of fast-tightening lipid pores due to chemotropic phase transition in the lipid bilayer. In this article, we continue studying this pore. We have found that Pal plus Ca(2+) permeabilize the plasma membrane of red blood cells in a dose-dependent manner. The same picture has been revealed for stearic acid (20 μM) but not for myristic and linoleic acids. The Pal-induced permeabilization of erythrocytic membranes can also occur in the presence of Ba(2+) and Mn(2+) (200 μM), but other bivalent cations (200 μM Mg(2+), Sr(2+), Ni(2+), Co(2+)) are relatively ineffective. The formation of Pal/Ca(2+)-induced pores in the erythrocytic membranes has been found to result in the destruction of cells.

Citing Articles

The Short-Term Opening of Cyclosporin A-Independent Palmitate/Sr-Induced Pore Can Underlie Ion Efflux in the Oscillatory Mode of Functioning of Rat Liver Mitochondria.

Belosludtseva N, Pavlik L, Belosludtsev K, Saris N, Shigaeva M, Mironova G Membranes (Basel). 2022; 12(7).

PMID: 35877870 PMC: 9319229. DOI: 10.3390/membranes12070667.

Anti-Diabetic and Antioxidant Activities of Red Wine Concentrate Enriched with Polyphenol Compounds under Experimental Diabetes in Rats.

Sabadashka M, Hertsyk D, Strugala-Danak P, Dudek A, Kanyuka O, Kucharska A Antioxidants (Basel). 2021; 10(9).

PMID: 34573031 PMC: 8471289. DOI: 10.3390/antiox10091399.

Mitochondrial Cyclosporine A-Independent Palmitate/Ca-Induced Permeability Transition Pore (PA-mPT Pore) and Its Role in Mitochondrial Function and Protection against Calcium Overload and Glutamate Toxicity.

Mironova G, Pavlov E Cells. 2021; 10(1).

PMID: 33440765 PMC: 7827677. DOI: 10.3390/cells10010125.

Effect of surface-potential modulators on the opening of lipid pores in liposomal and mitochondrial inner membranes induced by palmitate and calcium ions.

Belosludtsev K, Belosludtseva N, Agafonov A, Penkov N, Samartsev V, Lemasters J Biochim Biophys Acta. 2015; 1848(10 Pt A):2200-5.

PMID: 26014488 PMC: 4882158. DOI: 10.1016/j.bbamem.2015.05.013.

Interaction of phospholipase A of the E. coli outer membrane with the inhibitors of eucaryotic phospholipases A₂ and their effect on the Ca²⁺-induced permeabilization of the bacterial membrane.

Belosludtsev K, Belosludtseva N, Kondratyev M, Agafonov A, Purtov Y J Membr Biol. 2014; 247(3):281-8.

PMID: 24477786 DOI: 10.1007/s00232-014-9633-4.

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