Bone Regeneration in Surgically Created Defects Filled with Autogenous Bone: an Epifluorescence Microscopy Analysis in Rats

Overview

Journal J Appl Oral Sci

Specialty Dentistry

Date 2010 Sep 14

PMID 20835568

Citations 7

Authors

Marcos Heidy Guskuma

Eduardo Hochuli-Vieira

Flavia Priscila Pereira

Idelmo Rangel-Garcia Junior

Roberta Okamoto

Tetuo Okamoto

Osvaldo Magro Filho

Affiliations

Soon will be listed here.

Abstract

Unlabelled: Although the search for the ideal bone substitute has been the focus of a large number of studies, autogenous bone is still the gold standard for the filling of defects caused by pathologies and traumas, and mainly, for alveolar ridge reconstruction, allowing the titanium implants installation.

Objectives: The aim of this study was to evaluate the dynamics of autogenous bone graft incorporation process to surgically created defects in rat calvaria, using epifluorescence microscopy.

Material And Methods: Five adult male rats weighing 200-300 g were used. The animals received two 5-mm-diameter bone defects bilaterally in each parietal bone with a trephine bur under general anesthesia. Two groups of defects were formed: a control group (n=5), in which the defects were filled with blood clot, and a graft group (n=5), in which the defects were filled with autogenous bone block, removed from the contralateral defect. The fluorochromes calcein and alizarin were applied at the 7th and 30th postoperative days, respectively. The animals were killed at 35 days.

Results: The mineralization process was more intense in the graft group (32.09%) and occurred mainly between 7 and 30 days, the period labeled by calcein (24.66%).

Conclusions: The fluorochromes showed to be appropriate to label mineralization areas. The interfacial areas between fluorochrome labels are important sources of information about the bone regeneration dynamics.

Citing Articles

Preparation of Collagen/Hydroxyapatite Composites Using the Alternate Immersion Method and Evaluation of the Cranial Bone-Forming Capability of Composites Complexed with Acidic Gelatin and b-FGF.

Hoshi M, Taira M, Sawada T, Hachinohe Y, Hatakeyama W, Takafuji K Materials (Basel). 2022; 15(24).

PMID: 36556608 PMC: 9787395. DOI: 10.3390/ma15248802.

Vertical Guided Bone Regeneration in the Rabbit Calvarium Using Porous Nanohydroxyapatite Block Grafts Coated with rhVEGF and Cortical Perforation.

Liu W, Du B, Tan S, Wang Q, Li Y, Zhou L Int J Nanomedicine. 2020; 15:10059-10073.

PMID: 33335394 PMC: 7737884. DOI: 10.2147/IJN.S268182.

Dental stem cell sources and their potentials for bone tissue engineering.

Asutay F, Acar H, Yolcu U, Kirtay M, Alan H J Istanb Univ Fac Dent. 2017; 49(2):51-56.

PMID: 28955537 PMC: 5573487. DOI: 10.17096/jiufd.42908.

"Over-inlay" block graft and differential morphometry: a novel block graft model to study bone regeneration and host-to-graft interfaces in rats.

Ghiacci G, Graiani G, Ravanetti F, Lumetti S, Manfredi E, Galli C J Periodontal Implant Sci. 2016; 46(4):220-33.

PMID: 27588212 PMC: 5005810. DOI: 10.5051/jpis.2016.46.4.220.

Comparison of the Effect of Velvet Antler from Different Sections on Longitudinal Bone Growth of Adolescent Rats.

Kim H, Kim M, Leem K Evid Based Complement Alternat Med. 2016; 2016:1927534.

PMID: 27382403 PMC: 4921679. DOI: 10.1155/2016/1927534.

References

Pacifici L, Casella F, Ripari M . [The principles of tissue engineering: role of growth factors in the bone regeneration]. Minerva Stomatol. 2002; 51(9):351-9. View

Martin R . Label escape theory revisited: the effects of resting periods and section thickness. Bone. 1989; 10(4):255-64. DOI: 10.1016/8756-3282(89)90062-8. View

Triplett R, Schow S . Autologous bone grafts and endosseous implants: complementary techniques. J Oral Maxillofac Surg. 1996; 54(4):486-94. DOI: 10.1016/s0278-2391(96)90126-3. View

Costantino P, Hiltzik D, Govindaraj S, Moche J . Bone healing and bone substitutes. Facial Plast Surg. 2002; 18(1):13-26. DOI: 10.1055/s-2002-19823. View

Tadjoedin E, de Lange G, Lyaruu D, Kuiper L, Burger E . High concentrations of bioactive glass material (BioGran) vs. autogenous bone for sinus floor elevation. Clin Oral Implants Res. 2002; 13(4):428-36. DOI: 10.1034/j.1600-0501.2002.130412.x. View

Leite F, de Oliveira Ramalho L . Bone regeneration after demineralized bone matrix and castor oil (Ricinus communis) polyurethane implantation. J Appl Oral Sci. 2008; 16(2):122-6. PMC: 4327631. DOI: 10.1590/s1678-77572008000200008. View

Nkenke E, Schultze-Mosgau S, Radespiel-Troger M, Kloss F, Neukam F . Morbidity of harvesting of chin grafts: a prospective study. Clin Oral Implants Res. 2001; 12(5):495-502. DOI: 10.1034/j.1600-0501.2001.120510.x. View

Limeback H . Molecular mechanisms in dental hard tissue mineralization. Curr Opin Dent. 1991; 1(6):826-35. View

Branemark P, Lindstrom J, HALLEN O, BREINE U, Jeppson P, Ohman A . Reconstruction of the defective mandible. Scand J Plast Reconstr Surg. 1975; 9(2):116-28. DOI: 10.3109/02844317509022776. View

10.

OBrien F, Taylor D, Lee T . An improved labelling technique for monitoring microcrack growth in compact bone. J Biomech. 2002; 35(4):523-6. DOI: 10.1016/s0021-9290(01)00200-7. View

11.

Maniatopoulos C, Rodriguez A, Deporter D, Melcher A . An improved method for preparing histological sections of metallic implants. Int J Oral Maxillofac Implants. 1986; 1(1):31-7. View

12.

Busch O, Solheim E, Bang G, Tornes K . Guided tissue regeneration and local delivery of insulinlike growth factor I by bioerodible polyorthoester membranes in rat calvarial defects. Int J Oral Maxillofac Implants. 1996; 11(4):498-505. View

13.

Burchardt H . The biology of bone graft repair. Clin Orthop Relat Res. 1983; (174):28-42. View

14.

Zanchetta P, Lagarde N, Guezennec J . Systemic effects on bone healing of a new hyaluronic acid-like bacterial exopolysaccharide. Calcif Tissue Int. 2003; 73(3):232-6. DOI: 10.1007/s00223-002-2081-7. View

15.

Schlegel K, Lang F, Donath K, Kulow J, Wiltfang J . The monocortical critical size bone defect as an alternative experimental model in testing bone substitute materials. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006; 102(1):7-13. DOI: 10.1016/j.tripleo.2005.09.011. View

16.

Marx R, Carlson E, Eichstaedt R, Schimmele S, Strauss J, Georgeff K . Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998; 85(6):638-46. DOI: 10.1016/s1079-2104(98)90029-4. View

17.

Wood R, Moore D . Grafting of the maxillary sinus with intraorally harvested autogenous bone prior to implant placement. Int J Oral Maxillofac Implants. 1988; 3(3):209-14. View

18.

Hollinger J, Kleinschmidt J . The critical size defect as an experimental model to test bone repair materials. J Craniofac Surg. 1990; 1(1):60-8. DOI: 10.1097/00001665-199001000-00011. View

19.

Esteves J, Aranega A, Borrasca A, Fattah C, Garcia-Junior I . Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats. J Appl Oral Sci. 2008; 16(5):316-20. PMC: 4327596. DOI: 10.1590/s1678-77572008000500003. View

20.

Papalexiou V, Novaes Jr A, Grisi M, Souza S, Taba Jr M, Kajiwara J . Influence of implant microstructure on the dynamics of bone healing around immediate implants placed into periodontally infected sites. A confocal laser scanning microscopic study. Clin Oral Implants Res. 2004; 15(1):44-53. DOI: 10.1111/j.1600-0501.2004.00995.x. View