Sub1 Globally Regulates RNA Polymerase II C-terminal Domain Phosphorylation

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 2010 Sep 9

PMID 20823273

Citations 17

Authors

Alicia Garcia

Emanuel Rosonina

James L Manley

Olga Calvo

Affiliations

Soon will be listed here.

Abstract

The transcriptional coactivator Sub1 has been implicated in several aspects of mRNA metabolism in yeast, such as activation of transcription, termination, and 3'-end formation. Here, we present evidence that Sub1 plays a significant role in controlling phosphorylation of the RNA polymerase II large subunit C-terminal domain (CTD). We show that SUB1 genetically interacts with the genes encoding all four known CTD kinases, SRB10, KIN28, BUR1, and CTK1, suggesting that Sub1 acts to influence CTD phosphorylation at more than one step of the transcription cycle. To address this directly, we first used in vitro kinase assays, and we show that, on the one hand, SUB1 deletion increased CTD phosphorylation by Kin28, Bur1, and Ctk1 but, on the other, it decreased CTD phosphorylation by Srb10. Second, chromatin immunoprecipitation assays revealed that SUB1 deletion decreased Srb10 chromatin association on the inducible GAL1 gene but increased Kin28 and Ctk1 chromatin association on actively transcribed genes. Taken together, our data point to multiple roles for Sub1 in the regulation of CTD phosphorylation throughout the transcription cycle.

Citing Articles

Pif1 Helicase Mediates Remodeling of Protein-Nucleic Acid Complexes by Promoting Dissociation of Sub1 from G-Quadruplex DNA and Cdc13 from G-Rich Single-Stranded DNA.

Chib S, Griffin W, Gao J, Proffitt D, Byrd A, Raney K Biochemistry. 2023; 62(23):3360-3372.

PMID: 37948114 PMC: 10841737. DOI: 10.1021/acs.biochem.3c00441.

The Role of Sub1/PC4 in Transcription Elongation Depends on the C-Terminal Region and Is Independent of the ssDNA Binding Domain.

Collin A, Gonzalez-Jimenez A, Gonzalez-Jimenez M, Alfonso M, Calvo O Cells. 2022; 11(20).

PMID: 36291192 PMC: 9600219. DOI: 10.3390/cells11203320.

The Catalytic Subunit of CK2 (Cka1) Negatively Regulates RNA Polymerase II Transcription through Phosphorylation of Positive Cofactor 4 (PC4).

Rojas D, Urbina F, Solari A, Maldonado E Int J Mol Sci. 2022; 23(16).

PMID: 36012759 PMC: 9409219. DOI: 10.3390/ijms23169499.

Sub1 and RNAPII, until termination does them part.

Calvo O Transcription. 2017; 9(1):52-60.

PMID: 28853990 PMC: 5791812. DOI: 10.1080/21541264.2017.1333558.

Sub1/PC4, a multifaceted factor: from transcription to genome stability.

Garavis M, Calvo O Curr Genet. 2017; 63(6):1023-1035.

PMID: 28567479 DOI: 10.1007/s00294-017-0715-6.

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