Effects of Succinylated Concanavalin A on Infectivity and Syncytial Formation of Human Immunodeficiency Virus

Overview

Journal Med Microbiol Immunol

Specialty Allergy & Immunology

Date 1990 Jan 1

PMID 2082156

Citations 12

Authors

T Matsui

S Kobayashi

O Yoshida

S Ishii

Y Abe

N Yamamoto

Affiliations

Soon will be listed here.

Abstract

The effects of various lectins on the infectivity of human immunodeficiency virus (HIV) type 1 was investigated. Among the 25 lectins investigated, 2 types of concanavalin A (Con A) and 3 types of phytohemagglutinin were found to inhibit HIV infection. Succinylated Con A (S-Con A) efficiently blocked HIV-induced formation of syncytia in a coculture of MOLT-4 cells and blocked cell-free infection by HIV of MT-4 cells. The HIV-binding study revealed that S-Con A only partially inhibited viral binding to cells, although the control Leu-3a monoclonal antibody strongly inhibited it. When S-Con A was added to cultures after the initiation of viral adsorption, the number of HIV antigen-positive cells that developed depended on the time interval before addition of the compound. S-Con A inhibited HIV infection even after viral binding to cells at 0 degrees C and further incubation at 37 degrees C for 1 day. These data suggest that S-Con A inhibited mainly the fusion process rather than viral binding to cells in exerting its anti-HIV activity.

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