Early Diagnosis of Dengue Infection Using a Commercial Dengue Duo Rapid Test Kit for the Detection of NS1, IGM, and IGG

Overview

Journal Am J Trop Med Hyg

Specialty Tropical Medicine

Date 2010 Sep 3

PMID 20810840

Citations 70

Authors

Seok Mui Wang

Shamala Devi Sekaran

Affiliations

Soon will be listed here.

Abstract

A commercial Dengue Duo rapid test kit was evaluated for early dengue diagnosis by detection of dengue virus NS1 antigen and immunoglobulin M (IgM)/IgG antibodies. A total of 420 patient serum samples were subjected to real-time reverse transcription-polymerase chain reaction (RT-PCR), in-house IgM capture enzyme-linked immunosorbent assay (ELISA), hemagglutination inhibition assay, and the SD Dengue Duo rapid test. Of the 320 dengue acute and convalescent sera, dengue infection was detected by either serology or RT-PCR in 300 samples (93.75%), as compared with 289 samples (90.31%) in the combined SD Duo NS1/IgM. The NS1 detection rate is inversely proportional, whereas the IgM detection rate is directly proportional to the presence of IgG antibodies. The sensitivity and specificity in diagnosing acute dengue infection in the SD Duo NS1/IgM were 88.65% and 98.75%, respectively. The assay is sensitive and highly specific. Detection of both NS1 and IgM by SD Duo gave comparable detection rate by either serology or RT-PCR.

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References

Dussart P, Petit L, Labeau B, Bremand L, Leduc A, Moua D . Evaluation of two new commercial tests for the diagnosis of acute dengue virus infection using NS1 antigen detection in human serum. PLoS Negl Trop Dis. 2008; 2(8):e280. PMC: 2500180. DOI: 10.1371/journal.pntd.0000280. View

Kuberski T, Rosen L . A simple technique for the detection of dengue antigen in mosquitoes by immunofluorescence. Am J Trop Med Hyg. 1977; 26(3):533-7. DOI: 10.4269/ajtmh.1977.26.533. View

Kumarasamy V, Abdul Wahab A, Chua S, Hassan Z, Chem Y, Mohamad M . Evaluation of a commercial dengue NS1 antigen-capture ELISA for laboratory diagnosis of acute dengue virus infection. J Virol Methods. 2006; 140(1-2):75-9. DOI: 10.1016/j.jviromet.2006.11.001. View

Gubler D, Kuno G, SATHER G, Velez M, Oliver A . Mosquito cell cultures and specific monoclonal antibodies in surveillance for dengue viruses. Am J Trop Med Hyg. 1984; 33(1):158-65. DOI: 10.4269/ajtmh.1984.33.158. View

Gubler D, Meltzer M . Impact of dengue/dengue hemorrhagic fever on the developing world. Adv Virus Res. 1999; 53:35-70. DOI: 10.1016/s0065-3527(08)60342-5. View

Blacksell S, Mammen Jr M, Thongpaseuth S, Gibbons R, Jarman R, Jenjaroen K . Evaluation of the Panbio dengue virus nonstructural 1 antigen detection and immunoglobulin M antibody enzyme-linked immunosorbent assays for the diagnosis of acute dengue infections in Laos. Diagn Microbiol Infect Dis. 2007; 60(1):43-9. DOI: 10.1016/j.diagmicrobio.2007.07.011. View

Clarke D, Casals J . Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Am J Trop Med Hyg. 1958; 7(5):561-73. DOI: 10.4269/ajtmh.1958.7.561. View

Shu P, Huang J . Current advances in dengue diagnosis. Clin Diagn Lab Immunol. 2004; 11(4):642-50. PMC: 440621. DOI: 10.1128/CDLI.11.4.642-650.2004. View

McBride W . Evaluation of dengue NS1 test kits for the diagnosis of dengue fever. Diagn Microbiol Infect Dis. 2009; 64(1):31-6. DOI: 10.1016/j.diagmicrobio.2009.01.002. View

10.

. World Medical Association Declaration of Helsinki: ethical principles for medical research involving human subjects. JAMA. 2000; 284(23):3043-5. View

11.

Vajpayee M, Singh U, Seth P, Broor S . Comparative evaluation of various commercial assays for diagnosis of dengue fever. Southeast Asian J Trop Med Public Health. 2002; 32(3):472-5. View

12.

Young P, Hilditch P, Bletchly C, HALLORAN W . An antigen capture enzyme-linked immunosorbent assay reveals high levels of the dengue virus protein NS1 in the sera of infected patients. J Clin Microbiol. 2000; 38(3):1053-7. PMC: 86336. DOI: 10.1128/JCM.38.3.1053-1057.2000. View

13.

Lapphra K, Sangcharaswichai A, Chokephaibulkit K, Tiengrim S, Piriyakarnsakul W, Chakorn T . Evaluation of an NS1 antigen detection for diagnosis of acute dengue infection in patients with acute febrile illness. Diagn Microbiol Infect Dis. 2008; 60(4):387-91. DOI: 10.1016/j.diagmicrobio.2007.11.010. View

14.

Dussart P, Labeau B, Lagathu G, Louis P, Nunes M, Rodrigues S . Evaluation of an enzyme immunoassay for detection of dengue virus NS1 antigen in human serum. Clin Vaccine Immunol. 2006; 13(11):1185-9. PMC: 1656554. DOI: 10.1128/CVI.00229-06. View

15.

Yong Y, Thayan R, Chong H, Tan C, Sekaran S . Rapid detection and serotyping of dengue virus by multiplex RT-PCR and real-time SYBR green RT-PCR. Singapore Med J. 2007; 48(7):662-8. View

16.

Alcon S, Talarmin A, Debruyne M, Falconar A, Deubel V, Flamand M . Enzyme-linked immunosorbent assay specific to Dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections. J Clin Microbiol. 2002; 40(2):376-81. PMC: 153354. DOI: 10.1128/JCM.40.02.376-381.2002. View

17.

Lam S, Devi S, Pang T . Detection of specific IgM in dengue infection. Southeast Asian J Trop Med Public Health. 1987; 18(4):532-8. View