Cytosolic NADPH Balancing in Penicillium Chrysogenum Cultivated on Mixtures of Glucose and Ethanol

Overview

Journal Appl Microbiol Biotechnol

Specialties Biomedical Engineering
Biotechnology
Microbiology

Date 2010 Sep 3

PMID 20809073

Citations 2

Authors

Zheng Zhao

Karel Kuijvenhoven

Walter M van Gulik

Joseph J Heijnen

Wouter A van Winden

Peter J T Verheijen

Affiliations

Soon will be listed here.

Abstract

The in vivo flux through the oxidative branch of the pentose phosphate pathway (oxPPP) in Penicillium chrysogenum was determined during growth in glucose/ethanol carbon-limited chemostat cultures, at the same growth rate. Non-stationary (13)C flux analysis was used to measure the oxPPP flux. A nearly constant oxPPP flux was found for all glucose/ethanol ratios studied. This indicates that the cytosolic NADPH supply is independent of the amount of assimilated ethanol. The cofactor assignment in the model of van Gulik et al. (Biotechnol Bioeng 68(6):602-618, 2000) was supported using the published genome annotation of P. chrysogenum. Metabolic flux analysis showed that NADPH requirements in the cytosol remain nearly the same in these experiments due to constant biomass growth. Based on the cytosolic NADPH balance, it is known that the cytosolic aldehyde dehydrogenase in P. chrysogenum is NAD(+) dependent. Metabolic modeling shows that changing the NAD(+)-aldehyde dehydrogenase to NADP(+)-aldehyde dehydrogenase can increase the penicillin yield on substrate.

Citing Articles

NADPH-generating systems in bacteria and archaea.

Spaans S, Weusthuis R, van der Oost J, Kengen S Front Microbiol. 2015; 6:742.

PMID: 26284036 PMC: 4518329. DOI: 10.3389/fmicb.2015.00742.

Degeneration of penicillin production in ethanol-limited chemostat cultivations of Penicillium chrysogenum: A systems biology approach.

Douma R, Batista J, Touw K, Kiel J, Krikken A, Zhao Z BMC Syst Biol. 2011; 5:132.

PMID: 21854586 PMC: 3224390. DOI: 10.1186/1752-0509-5-132.

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