Crystal Structure of Human Adenovirus at 3.5 A Resolution

Overview

Journal Science

Specialty Science

Date 2010 Aug 28

PMID 20798318

Citations 114

Authors

Vijay S Reddy

S Kundhavai Natchiar

Phoebe L Stewart

Glen R Nemerow

Affiliations

Soon will be listed here.

Abstract

Rational development of adenovirus vectors for therapeutic gene transfer is hampered by the lack of accurate structural information. Here, we report the x-ray structure at 3.5 angstrom resolution of the 150-megadalton adenovirus capsid containing nearly 1 million amino acids. We describe interactions between the major capsid protein (hexon) and several accessory molecules that stabilize the capsid. The virus structure also reveals an altered association between the penton base and the trimeric fiber protein, perhaps reflecting an early event in cell entry. The high-resolution structure provides a substantial advance toward understanding the assembly and cell entry mechanisms of a large double-stranded DNA virus and provides new opportunities for improving adenovirus-mediated gene transfer.

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