Analysis of Caprine Mycoplasmas and Mycoplasma Infections in Goats Using Two-dimensional Electrophoresis and Immunoblotting

Overview

Journal Electrophoresis

Specialty Chemistry

Date 1990 Oct 1

PMID 2079028

Citations 6

Authors

B Olsson

G Bolske

K Bergstrom

K E Johansson

Affiliations

Soon will be listed here.

Abstract

Seven strains of mycoplasma have been characterized by two-dimensional polyacrylamide gel electrophoresis in combination with immunoblotting. The two-dimensional patterns of the strains were compared and similarities and differences are discussed. Hyperimmune rabbit antisera were used in the immunoblotting experiment to study the immunological cross-reactivity between the species. Sera from goats, naturally and experimentally infected with mycoplasma, were also used in the immunoblotting experiment to study the immune responses of the infected animals.

Citing Articles

Experimental contagious caprine pleuropneumonia: a long term study on the course of infection and pathology in a flock of goats infected with Mycoplasma capricolum subsp. capripneumoniae.

Wesonga H, Bolske G, Thiaucourt F, Wanjohi C, Lindberg R Acta Vet Scand. 2005; 45(3-4):167-79.

PMID: 15663077 PMC: 1820987. DOI: 10.1186/1751-0147-45-167.

Genetic and serological analysis of lipoprotein LppA in Mycoplasma mycoides subsp. mycoides LC and Mycoplasma mycoides subsp. capri.

Monnerat M, Thiaucourt F, Poveda J, Nicolet J, Frey J Clin Diagn Lab Immunol. 1999; 6(2):224-30.

PMID: 10066658 PMC: 95691. DOI: 10.1128/CDLI.6.2.224-230.1999.

Molecular evolution of Mycoplasma capricolum subsp. capripneumoniae strains, based on polymorphisms in the 16S rRNA genes.

Pettersson B, Bolske G, Thiaucourt F, Uhlen M, Johansson K J Bacteriol. 1998; 180(9):2350-8.

PMID: 9573185 PMC: 107175. DOI: 10.1128/JB.180.9.2350-2358.1998.

Contagious bovine pleuropneumonia in northern Tanzania, culture confirmation and serological studies.

Bolske G, Msami H, Gunnarsson A, Kapaga A, Loomu P Trop Anim Health Prod. 1995; 27(4):193-201.

PMID: 8966759 DOI: 10.1007/BF02250690.

Diagnosis of contagious caprine pleuropneumonia by detection and identification of Mycoplasma capricolum subsp. capripneumoniae by PCR and restriction enzyme analysis.

Bolske G, Mattsson J, Bascunana C, Bergstrom K, Wesonga H, Johansson K J Clin Microbiol. 1996; 34(4):785-91.

PMID: 8815084 PMC: 228893. DOI: 10.1128/jcm.34.4.785-791.1996.