Control of Protein Synthesis in Extracts from Poliovirus-infected Cells. I. MRNA Discrimination by Crude Initiation Factors

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1978 May 1

PMID 207899

Citations 37

Authors

T Helentjaris

E EHRENFELD

Affiliations

Soon will be listed here.

Abstract

By using cell-free systems prepared from uninfected and poliovirus-infected cells, we have been able to demonstrate that crude preparations of initiation factors from infected cells do not stimulate the initiation of translation by polyribosomes containing endogenous host cell mRNA. When tested with polysomes containing endogenous viral mRNA, however, they were able to stimulate initiation of translation nearly as well as uninfected cell initiation factors. The uninfected cell initiation factor preparations were able to stimulate initiation of translation of both cell and viral mRNA. The results indicate an mRNA-specific activity present in crude initiation factor preparations from infected cells. Furthermore, the ability of eIF2 from infected cells to form a ternary complex with GTP and formyl [35S]methionine-tRNAfmet, an mRNA-independent step in initiation, was found not to be deficient. Implications of these data for proposed mechanisms of poliovirus-induced host cell shutoff are discussed.

Citing Articles

Involvement of a Nonstructural Protein in Poliovirus Capsid Assembly.

Adeyemi O, Sherry L, Ward J, Pierce D, Herod M, Rowlands D J Virol. 2018; 93(5).

PMID: 30541849 PMC: 6384072. DOI: 10.1128/JVI.01447-18.

The multifaceted poliovirus 2A protease: regulation of gene expression by picornavirus proteases.

Castello A, Alvarez E, Carrasco L J Biomed Biotechnol. 2011; 2011:369648.

PMID: 21541224 PMC: 3085340. DOI: 10.1155/2011/369648.

mTOR-dependent stimulation of the association of eIF4G and eIF3 by insulin.

Harris T, Chi A, Shabanowitz J, Hunt D, Rhoads R, Lawrence Jr J EMBO J. 2006; 25(8):1659-68.

PMID: 16541103 PMC: 1440840. DOI: 10.1038/sj.emboj.7601047.

Localization of cap-binding protein in subcellular fractions of HeLa cells.

Hansen J, Etchison D, Hershey J, EHRENFELD E Mol Cell Biol. 1982; 2(12):1639-43.

PMID: 14582205 PMC: 369972. DOI: 10.1128/mcb.2.12.1639-1643.1982.

Resistance of ribosomal protein mRNA translation to protein synthesis shutoff induced by poliovirus.

Cardinali B, Fiore L, Campioni N, De Dominicis A J Virol. 1999; 73(8):7070-6.

PMID: 10400812 PMC: 112799. DOI: 10.1128/JVI.73.8.7070-7076.1999.

References

Golini F, Thach S, BIRGE C, Safer B, Merrick W, Thach R . Competition between cellular and viral mRNAs in vitro is regulated by a messenger discriminatory initiation factor. Proc Natl Acad Sci U S A. 1976; 73(9):3040-4. PMC: 430920. DOI: 10.1073/pnas.73.9.3040. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Nomoto A, Detjen B, Pozzatti R, Wimmer E . The location of the polio genome protein in viral RNAs and its implication for RNA synthesis. Nature. 1977; 268(5617):208-13. DOI: 10.1038/268208a0. View

EHRENFELD E, Lund H . Untranslated vesicular stomatitis virus messenger RNA after poliovirus infection. Virology. 1977; 80(2):297-308. DOI: 10.1016/s0042-6822(77)80006-8. View

Helentjaris T, EHRENFELD E . Inhibition of host cell protein synthesis by UV-inactivated poliovirus. J Virol. 1977; 21(1):259-67. PMC: 353811. DOI: 10.1128/JVI.21.1.259-267.1977. View

Blair G, Dahl H, TRUELSEN E, LELONG J . Functional identity of a mouse ascites and a rabbit reticulocyte initiation factor required for natural mRNA translation. Nature. 1977; 265(5595):651-3. DOI: 10.1038/265651a0. View

Colby D, Finnerty V . Fate of mRNA of L-cells infected with mengovirus. J Virol. 1974; 13(4):858-69. PMC: 355384. DOI: 10.1128/JVI.13.4.858-869.1974. View

Wright P, Cooper P . Poliovirus proteins associated with ribosomal structures in infected cells. Virology. 1974; 59(1):1-20. DOI: 10.1016/0042-6822(74)90201-3. View

Celma M, EHRENFELD E . Effect of poliovirus double-stranded RNA on viral and host-cell protein synthesis. Proc Natl Acad Sci U S A. 1974; 71(6):2440-4. PMC: 388473. DOI: 10.1073/pnas.71.6.2440. View

10.

Cole C, Baltimore D . Defective interfering particles of poliovirus. II. Nature of the defect. J Mol Biol. 1973; 76(3):325-43. DOI: 10.1016/0022-2836(73)90508-1. View

11.

Smith A, Marcker K . Cytoplasmic methionine transfer RNAs from eukaryotes. Nature. 1970; 226(5246):607-10. DOI: 10.1038/226607a0. View

12.

Willems M, Penman S . The mechanism of host cell protein synthesis inhibition by poliovirus. Virology. 1966; 30(3):355-67. DOI: 10.1016/0042-6822(66)90114-0. View

13.

Both G, Furuichi Y, Muthukrishnan S, Shatkin A . Ribosome binding to reovirus mRNA in protein synthesis requires 5' terminal 7-methylguanosine. Cell. 1975; 6(2):185-95. DOI: 10.1016/0092-8674(75)90009-4. View

14.

Nuss D, Oppermann H, Koch G . Selective blockage of initiation of host protein synthesis in RNA-virus-infected cells. Proc Natl Acad Sci U S A. 1975; 72(4):1258-62. PMC: 432511. DOI: 10.1073/pnas.72.4.1258. View

15.

Celma M, EHRENFELD E . Translation of poliovirus RNA in vitro: detection of two different initiation sites. J Mol Biol. 1975; 98(4):761-80. DOI: 10.1016/s0022-2836(75)80009-x. View

16.

Hewlett M, Rose J, Baltimore D . 5'-terminal structure of poliovirus polyribosomal RNA is pUp. Proc Natl Acad Sci U S A. 1976; 73(2):327-30. PMC: 335900. DOI: 10.1073/pnas.73.2.327. View

17.

Abreu S . Cellular protein synthesis shutoff by mengovirus: translation of nonviral and viral mRNA's in extracts from uninfected and infected Ehrlich ascites tumor cells. J Virol. 1976; 18(1):182-94. PMC: 515537. DOI: 10.1128/JVI.18.1.182-194.1976. View

18.

Bonner W, Laskey R . A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974; 46(1):83-8. DOI: 10.1111/j.1432-1033.1974.tb03599.x. View

19.

Capecchi M . Initiation of E. coli proteins. Proc Natl Acad Sci U S A. 1966; 55(6):1517-24. PMC: 224353. DOI: 10.1073/pnas.55.6.1517. View

20.

Shafritz D, Weinstein J, Safer B, Merrick W, Weber L, Hickey E . Evidence for role of m7G5'-phosphate group in recognition of eukaryotic mRNA by initiation factor IF-M3. Nature. 1976; 261(5558):291-4. DOI: 10.1038/261291a0. View