Protein Polymer MRI Contrast Agents: Longitudinal Analysis of Biomaterials in Vivo

Overview

Journal Magn Reson Med

Publisher Wiley

Specialty Radiology

Date 2010 Aug 27

PMID 20740653

Citations 9

Authors

Lindsay S Karfeld-Sulzer

Emily A Waters

Ellen K Kohlmeir

Hermann Kissler

Xiaomin Zhang

Dixon B Kaufman

Annelise E Barron

Thomas J Meade

Affiliations

Soon will be listed here.

Abstract

Despite recent advances in tissue engineering to regenerate biological function by combining cells with material supports, development is hindered by inadequate techniques for characterizing biomaterials in vivo. Magnetic resonance imaging is a tomographic technique with high temporal and spatial resolution and represents an excellent imaging modality for longitudinal noninvasive assessment of biomaterials in vivo. To distinguish biomaterials from surrounding tissues for magnetic resonance imaging, protein polymer contrast agents were developed and incorporated into hydrogels. In vitro and in vivo images of protein polymer hydrogels, with and without covalently incorporated protein polymer contrast agents, were acquired by magnetic resonance imaging. T(1) values of the labeled gels were consistently lower when protein polymer contrast agents were included. As a result, the protein polymer contrast agent hydrogels facilitated fate tracking, quantification of degradation, and detection of immune response in vivo. For the duration of the in vivo study, the protein polymer contrast agent-containing hydrogels could be distinguished from adjacent tissues and from the foreign body response surrounding the gels. The hydrogels containing protein polymer contrast agent have a contrast-to-noise ratio 2-fold greater than hydrogels without protein polymer contrast agent. In the absence of the protein polymer contrast agent, hydrogels cannot be distinguished by the end of the gel lifetime.

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References

Cheng H, Wallis C, Shou Z, Farhat W . Quantifying angiogenesis in VEGF-enhanced tissue-engineered bladder constructs by dynamic contrast-enhanced MRI using contrast agents of different molecular weights. J Magn Reson Imaging. 2006; 25(1):137-45. DOI: 10.1002/jmri.20787. View

Zhou X, Westlund P . The viscosity and temperature dependence of (1)H T(1)-NMRD of the Gd(H(2)O)(8)(3+) complex. Spectrochim Acta A Mol Biomol Spectrosc. 2005; 62(1-3):335-42. DOI: 10.1016/j.saa.2004.12.051. View

Kong H, Kaigler D, Kim K, Mooney D . Controlling rigidity and degradation of alginate hydrogels via molecular weight distribution. Biomacromolecules. 2004; 5(5):1720-7. DOI: 10.1021/bm049879r. View

Miyata S, Numano T, Homma K, Tateishi T, Ushida T . Feasibility of noninvasive evaluation of biophysical properties of tissue-engineered cartilage by using quantitative MRI. J Biomech. 2007; 40(13):2990-8. DOI: 10.1016/j.jbiomech.2007.02.002. View

Davis N, Ding S, Forster R, Pinkas D, Barron A . Modular enzymatically crosslinked protein polymer hydrogels for in situ gelation. Biomaterials. 2010; 31(28):7288-97. PMC: 3286757. DOI: 10.1016/j.biomaterials.2010.06.003. View

Jaszberenyi Z, Moriggi L, Schmidt P, Weidensteiner C, Kneuer R, Merbach A . Physicochemical and MRI characterization of Gd3+-loaded polyamidoamine and hyperbranched dendrimers. J Biol Inorg Chem. 2007; 12(3):406-20. DOI: 10.1007/s00775-006-0197-3. View

Mader K, Bacic G, Domb A, Elmalak O, Langer R, Swartz H . Noninvasive in vivo monitoring of drug release and polymer erosion from biodegradable polymers by EPR spectroscopy and NMR imaging. J Pharm Sci. 1997; 86(1):126-34. DOI: 10.1021/js9505105. View

Wang X, Feng Y, Ke T, Schabel M, Lu Z . Pharmacokinetics and tissue retention of (Gd-DTPA)-cystamine copolymers, a biodegradable macromolecular magnetic resonance imaging contrast agent. Pharm Res. 2005; 22(4):596-602. DOI: 10.1007/s11095-005-2489-7. View

Weissleder R, Poss K, Wilkinson R, Zhou C, Bogdanov Jr A . Quantitation of slow drug release from an implantable and degradable gentamicin conjugate by in vivo magnetic resonance imaging. Antimicrob Agents Chemother. 1995; 39(4):839-45. PMC: 162639. DOI: 10.1128/AAC.39.4.839. View

10.

Huber M, Staubli A, Kustedjo K, Gray M, Shih J, Fraser S . Fluorescently detectable magnetic resonance imaging agents. Bioconjug Chem. 1998; 9(2):242-9. DOI: 10.1021/bc970153k. View

11.

Peptan I, Hong L, Xu H, Magin R . MR assessment of osteogenic differentiation in tissue-engineered constructs. Tissue Eng. 2006; 12(4):843-51. DOI: 10.1089/ten.2006.12.843. View

12.

Langer R, Tirrell D . Designing materials for biology and medicine. Nature. 2004; 428(6982):487-92. DOI: 10.1038/nature02388. View

13.

Liu S, Tobias R, McClure S, Styba G, Shi Q, Jackowski G . Removal of endotoxin from recombinant protein preparations. Clin Biochem. 1997; 30(6):455-63. DOI: 10.1016/s0009-9120(97)00049-0. View

14.

Mikos , McIntire , Anderson , Babensee . Host response to tissue engineered devices. Adv Drug Deliv Rev. 2000; 33(1-2):111-139. DOI: 10.1016/s0169-409x(98)00023-4. View

15.

Meinel L, Hofmann S, Karageorgiou V, Zichner L, Langer R, Kaplan D . Engineering cartilage-like tissue using human mesenchymal stem cells and silk protein scaffolds. Biotechnol Bioeng. 2004; 88(3):379-91. DOI: 10.1002/bit.20252. View

16.

Mohs A, Lu Z . Gadolinium(III)-based blood-pool contrast agents for magnetic resonance imaging: status and clinical potential. Expert Opin Drug Deliv. 2007; 4(2):149-64. DOI: 10.1517/17425247.4.2.149. View

17.

Traore A, Woerly S, Doan V, Marois Y, Guidoin R . In vivo magnetic resonance imaging and relaxometry study of a porous hydrogel implanted in the trapezius muscle of rabbits. Tissue Eng. 2000; 6(3):265-78. DOI: 10.1089/10763270050044443. View

18.

Karfeld-Sulzer L, Waters E, Davis N, Meade T, Barron A . Multivalent protein polymer MRI contrast agents: controlling relaxivity via modulation of amino acid sequence. Biomacromolecules. 2010; 11(6):1429-36. PMC: 2892858. DOI: 10.1021/bm901378a. View

19.

Chen X, Astary G, Sepulveda H, Mareci T, Sarntinoranont M . Quantitative assessment of macromolecular concentration during direct infusion into an agarose hydrogel phantom using contrast-enhanced MRI. Magn Reson Imaging. 2008; 26(10):1433-41. PMC: 3140426. DOI: 10.1016/j.mri.2008.04.011. View

20.

Stroman P, Dorvil J, Marois Y, Poddevin N, Guidoin R . In vivo time course studies of the tissue responses to resorbable polylactic acid implants by means of MRI. Magn Reson Med. 1999; 42(1):210-4. DOI: 10.1002/(sici)1522-2594(199907)42:1<210::aid-mrm29>3.0.co;2-q. View