Dietary Olive Oil Effect on Antioxidant Status and Fatty Acid Profile in the Erythrocyte of 2,4-D- Exposed Rats

Overview

Journal Lipids Health Dis

Publisher Biomed Central

Specialties Biochemistry
Endocrinology

Date 2010 Aug 27

PMID 20738870

Citations 6

Authors

Amel Nakbi

Wafa Tayeb

Samia Dabbou

Manel Issaoui

Abir K Grissa

Nabil Attia

Mohamed Hammami

Affiliations

Soon will be listed here.

Abstract

Background: Oxidative stress produced by reactive oxygen species (ROS) has been linked to the development of several diseases such as cardiovascular, cancer, and neurodegenerative diseases. This study investigates the possible protective effect of extra virgin olive oil (EVOO), lipophilic fraction (OOLF) and hydrophilic fraction (OOHF) on oxidative stress and fatty acid profile of erythrocytes in 2,4-D treated rats.

Methods: Male Wistar rats were divided randomly into eight groups: control (C), (2,4-D) at a dose of 5 mg/kg b.w., (2,4-D/EVOO) was given 2,4-D plus EVOO, (2,4-D/OOHF) that received 2,4-D plus hydrophilic fraction, (2,4-D/OOLF) treated with 2,4-D plus lipophilic fraction, (EVOO) that received only EVOO, (OOHF) was given hydrophilic fraction and (OOLF) treated with lipophilic fraction. These components were daily administered by gavages for 4 weeks.

Results: 2,4-D treatment lead to decrease of antioxidant enzyme activities, namely, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR) associated with a higher amount of MDA level. Erythrocyte membranes' fatty acid composition was also significantly modified with 2,4-D exposure. EVOO and hydrophilic fraction supplemented to rats with or not 2,4-D treatment enhanced the antioxidant enzyme activities and reduced the MDA level. However, lipophilic fraction did not show any improvement in oxidative damage induced by 2,4-D in spite its richness in MUFA and vitamins.

Conclusion: EVOO administered to 2,4-D-treated rats protected erythrocyte membranes against oxidative damage by means of preventing excessive lipid peroxidation to increase the MUFA composition and increase maintaining antioxidants enzymes at normal concentrations.

Citing Articles

Olive Oil Improves While Trans Fatty Acids Further Aggravate the Hypomethylation of LINE-1 Retrotransposon DNA in an Environmental Carcinogen Model.

Szabo L, Molnar R, Tomesz A, Deutsch A, Darago R, Varjas T Nutrients. 2022; 14(4).

PMID: 35215560 PMC: 8878525. DOI: 10.3390/nu14040908.

In vivo effects of olive oil and trans-fatty acids on miR-134, miR-132, miR-124-1, miR-9-3 and mTORC1 gene expression in a DMBA-treated mouse model.

Molnar R, Szabo L, Tomesz A, Deutsch A, Darago R, Ghodratollah N PLoS One. 2021; 16(2):e0246022.

PMID: 33539381 PMC: 7861522. DOI: 10.1371/journal.pone.0246022.

Current perspectives of oleic acid: Regulation of molecular pathways in mitochondrial and endothelial functioning against insulin resistance and diabetes.

Rehman K, Haider K, Jabeen K, Akash M Rev Endocr Metab Disord. 2020; 21(4):631-643.

PMID: 32125563 DOI: 10.1007/s11154-020-09549-6.

Toxicological Effects of Traumatic Acid and Selected Herbicides on Human Breast Cancer Cells: In Vitro Cytotoxicity Assessment of Analyzed Compounds.

Jablonska-Trypuc A, Wydro U, Wolejko E, Butarewicz A Molecules. 2019; 24(9).

PMID: 31052542 PMC: 6539929. DOI: 10.3390/molecules24091710.

Protective Effects of α-Tocopherol, γ-Tocopherol and Oleic Acid, Three Compounds of Olive Oils, and No Effect of Trolox, on 7-Ketocholesterol-Induced Mitochondrial and Peroxisomal Dysfunction in Microglial BV-2 Cells.

Debbabi M, Nury T, Zarrouk A, Mekahli N, Bezine M, Sghaier R Int J Mol Sci. 2016; 17(12).

PMID: 27897980 PMC: 5187773. DOI: 10.3390/ijms17121973.

References

Lee C, Barnett J, Reaven P . Liposomes enriched in oleic acid are less susceptible to oxidation and have less proinflammatory activity when exposed to oxidizing conditions. J Lipid Res. 1998; 39(6):1239-47. View

Aviram M . Interaction of oxidized low density lipoprotein with macrophages in atherosclerosis, and the antiatherogenicity of antioxidants. Eur J Clin Chem Clin Biochem. 1996; 34(8):599-608. View

Li Z, Berk M, McIntyre T, Feldstein A . Hepatic lipid partitioning and liver damage in nonalcoholic fatty liver disease: role of stearoyl-CoA desaturase. J Biol Chem. 2009; 284(9):5637-44. PMC: 2645822. DOI: 10.1074/jbc.M807616200. View

Halliwell B . Antioxidants and human disease: a general introduction. Nutr Rev. 1997; 55(1 Pt 2):S44-9; discussion S49-52. DOI: 10.1111/j.1753-4887.1997.tb06100.x. View

Kyle M, Miccadei S, Nakae D, Farber J . Superoxide dismutase and catalase protect cultured hepatocytes from the cytotoxicity of acetaminophen. Biochem Biophys Res Commun. 1987; 149(3):889-96. DOI: 10.1016/0006-291x(87)90491-8. View

Massaro M, De Caterina R . Vasculoprotective effects of oleic acid: epidemiological background and direct vascular antiatherogenic properties. Nutr Metab Cardiovasc Dis. 2002; 12(1):42-51. View

Ginsberg H, Barr S, Gilbert A, Karmally W, Deckelbaum R, Kaplan K . Reduction of plasma cholesterol levels in normal men on an American Heart Association Step 1 diet or a Step 1 diet with added monounsaturated fat. N Engl J Med. 1990; 322(9):574-9. DOI: 10.1056/NEJM199003013220902. View

Visioli F, Galli C . Biological properties of olive oil phytochemicals. Crit Rev Food Sci Nutr. 2002; 42(3):209-21. DOI: 10.1080/10408690290825529. View

Escobar J, Rubio M, Lissi E . Sod and catalase inactivation by singlet oxygen and peroxyl radicals. Free Radic Biol Med. 1996; 20(3):285-90. DOI: 10.1016/0891-5849(95)02037-3. View

10.

Esterbauer H, Schaur R, Zollner H . Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. Free Radic Biol Med. 1991; 11(1):81-128. DOI: 10.1016/0891-5849(91)90192-6. View

11.

AEBI H . Catalase in vitro. Methods Enzymol. 1984; 105:121-6. DOI: 10.1016/s0076-6879(84)05016-3. View

12.

Gimeno E, Castellote A, Lamuela-Raventos R, de la Torre M, Lopez-Sabater M . Rapid determination of vitamin E in vegetable oils by reversed-phase high-performance liquid chromatography. J Chromatogr A. 2000; 881(1-2):251-4. DOI: 10.1016/s0021-9673(00)00219-3. View

13.

Bello R, Gomez-Diaz C, Buron M, Navas P, Villalba J . Differential regulation of hepatic apoptotic pathways by dietary olive and sunflower oils in the aging rat. Exp Gerontol. 2006; 41(11):1174-84. DOI: 10.1016/j.exger.2006.08.012. View

14.

Duchnowicz P, Koter M . Damage to the erythrocyte membrane caused by chlorophenoxyacetic herbicides. Cell Mol Biol Lett. 2003; 8(1):25-30. View

15.

Kandaswami C, MIDDLETON Jr E . Free radical scavenging and antioxidant activity of plant flavonoids. Adv Exp Med Biol. 1994; 366:351-76. DOI: 10.1007/978-1-4615-1833-4_25. View

16.

Bonanome A, Pagnan A, Biffanti S, Opportuno A, Sorgato F, Dorella M . Effect of dietary monounsaturated and polyunsaturated fatty acids on the susceptibility of plasma low density lipoproteins to oxidative modification. Arterioscler Thromb. 1992; 12(4):529-33. DOI: 10.1161/01.atv.12.4.529. View

17.

Kono Y, Fridovich I . Superoxide radical inhibits catalase. J Biol Chem. 1982; 257(10):5751-4. View

18.

Bukowska B . Effects of 2,4-D and its metabolite 2,4-dichlorophenol on antioxidant enzymes and level of glutathione in human erythrocytes. Comp Biochem Physiol C Toxicol Pharmacol. 2003; 135(4):435-41. DOI: 10.1016/s1532-0456(03)00151-0. View

19.

Teixeira M, Telo J, Duarte N, Sa-Correia I . The herbicide 2,4-dichlorophenoxyacetic acid induces the generation of free-radicals and associated oxidative stress responses in yeast. Biochem Biophys Res Commun. 2004; 324(3):1101-7. DOI: 10.1016/j.bbrc.2004.09.158. View

20.

Reaven P, Parthasarathy S, Grasse B, Miller E, Steinberg D, Witztum J . Effects of oleate-rich and linoleate-rich diets on the susceptibility of low density lipoprotein to oxidative modification in mildly hypercholesterolemic subjects. J Clin Invest. 1993; 91(2):668-76. PMC: 288008. DOI: 10.1172/JCI116247. View