Extracellular Matrices for Gastrointestinal Surgery: Ex Vivo Testing and Current Applications

Overview

Journal World J Gastroenterol

Publisher Baishideng Publishing Group

Specialty Gastroenterology

Date 2010 Aug 24

PMID 20731016

Citations 2

Authors

Jens Hoeppner

Goran Marjanovic

Peter Helwig

Ulrich Theodor Hopt

Tobias Keck

Affiliations

Soon will be listed here.

Abstract

Aim: To assess the effects of bile and pancreatic juice on structural and mechanical resistance of extracellular matrices (ECMs) in vitro.

Methods: Small-intestinal submucosa (SIS), porcine dermal matrix (PDM), porcine pericardial matrix (PPM) and bovine pericardial matrix (BPM) were incubated in human bile and pancreatic juice in vitro. ECMs were examined by macroscopic observation, scanning electron microscopy (SEM) and testing of mechanical resistance.

Results: PDM dissolved within 4 d after exposure to bile or pancreatic juice. SIS, PPM and PDM retained their integrity for > 60 d when incubated in either digestive juice. The effect of bile was found to be far more detrimental to mechanical stability than pancreatic juice in all tested materials. In SIS, the loss of mechanical stability after incubation in either of the digestive secretions was less distinct than in PPM and BPM [mFmax 4.01/14.27 N (SIS) vs 2.08/5.23 N (PPM) vs 1.48/7.89 N (BPM)]. In SIS, the extent of structural damage revealed by SEM was more evident in bile than in pancreatic juice. In PPM and BPM, structural damage was comparable in both media.

Conclusion: PDM is less suitable for support of gastrointestinal healing. Besides SIS, PPM and BPM should also be evaluated experimentally for gastrointestinal indications.

Citing Articles

The Contribution of a Collagen Patch Coated With Fibrin Glue in Sealing Upper Gastrointestinal Defects: An Experimental Study.

Christodoulidis G, Samara A, Floros T, Karakantas K, Zotos P, Koutras A In Vivo. 2021; 35(5):2697-2702.

PMID: 34410958 PMC: 8408736. DOI: 10.21873/invivo.12553.

Repair of gastric defects with an equine pericardial patch.

Spiliopoulos K, Markakis C, Tomos P, Gakiopoulou H, Nikolopoulos I, Spartalis E Surg Today. 2014; 45(1):83-90.

PMID: 25380578 DOI: 10.1007/s00595-014-1072-4.

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