Versican G1 Domain and V3 Isoform Overexpression Results in Increased Chondrogenesis in the Developing Chick Limb in Ovo

Overview

Journal Anat Rec (Hoboken)

Specialty Anatomy & Histology

Date 2010 Aug 24

PMID 20730861

Citations 6

Authors

Karla S Hudson

Kristen Andrews

June Early

Corey H Mjaatvedt

Anthony A Capehart

Affiliations

Soon will be listed here.

Abstract

Previous work has shown that versican proteoglycan is highly expressed in the extracellular matrix of precartilage limb mesenchyme. Although much of versican's role in chondrogenesis has been attributed to its glycosaminoglycan complement, N- and C-terminal G1 and G3 domains of versican have been shown to possess distinct functions when expressed ectopically. This study was undertaken to test the hypothesis that overexpression of the versican G1 domain and short V3 isoform, comprised of only G1 and G3, in the chick wing in ovo would result in increased chondrogenesis, suggesting function for discrete versican domains in limb skeletal development. Recombinant adenoviruses encoding G1 and V3 proteins were microinjected into proximal HH19-25 chick wing buds which resulted in significant enlargement of humeral primordia at HH35. Enhanced cartilage deposition appeared due to increased chondrogenic aggregation as a result of recombinant G1 or V3 overexpression, further implicating versican in early stages of limb development.

Citing Articles

V3: an enigmatic isoform of the proteoglycan versican.

Wight T, Day A, Kang I, Harten I, Kaber G, Briggs D Am J Physiol Cell Physiol. 2023; 325(2):C519-C537.

PMID: 37399500 PMC: 10511178. DOI: 10.1152/ajpcell.00059.2023.

Versican G1 domain enhances adenoviral-mediated transgene expression and can be modulated by inhibitors of the Janus kinase (JAK)/STAT and Src family kinase pathways.

Akinfenwa P, Bond W, Ildefonso C, Hurwitz M, Hurwitz R J Biol Chem. 2017; 292(35):14381-14390.

PMID: 28684419 PMC: 5582833. DOI: 10.1074/jbc.M116.773549.

Targeted In Situ Biosynthetic Transcriptional Activation in Native Surface-Level Human Articular Chondrocytes during Lesion Stabilization.

Ganguly K, McRury I, Goodwin P, Morgan R, Auge 2nd W Cartilage. 2015; 3(2):141-55.

PMID: 26069627 PMC: 4297128. DOI: 10.1177/1947603511426881.

Extracellular processing of the cartilage proteoglycan aggregate and its effect on CD44-mediated internalization of hyaluronan.

Danielson B, Knudson C, Knudson W J Biol Chem. 2015; 290(15):9555-70.

PMID: 25733665 PMC: 4392260. DOI: 10.1074/jbc.M115.643171.

Versican knockdown reduces interzone area during early stages of chick synovial joint development.

Nagchowdhuri P, Andrews K, Robart S, Capehart A Anat Rec (Hoboken). 2011; 295(3):397-409.

PMID: 22190409 PMC: 3339409. DOI: 10.1002/ar.21542.

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