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Generation of Recombinant Influenza Virus from Plasmid DNA

Overview
Journal J Vis Exp
Specialties Biology
General Medicine
Date 2010 Aug 24
PMID 20729804
Citations 87
Authors
Luis Martinez-Sobrido
Adolfo Garcia-Sastre
Affiliations
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Abstract

Efforts by a number of influenza research groups have been pivotal in the development and improvement of influenza A virus reverse genetics. Originally established in 1999 plasmid-based reverse genetic techniques to generate recombinant viruses have revolutionized the influenza research field because specific questions have been answered by genetically engineered, infectious, recombinant influenza viruses. Such studies include virus replication, function of viral proteins, the contribution of specific mutations in viral proteins in viral replication and/or pathogenesis and, also, viral vectors using recombinant influenza viruses expressing foreign proteins.

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References
1.
Schickli J, Flandorfer A, Nakaya T, Martinez-Sobrido L, Garcia-Sastre A, Palese P . Plasmid-only rescue of influenza A virus vaccine candidates. Philos Trans R Soc Lond B Biol Sci. 2002; 356(1416):1965-73. PMC: 1088576. DOI: 10.1098/rstb.2001.0979. View
2.
Niwa H, Yamamura K, Miyazaki J . Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene. 1991; 108(2):193-9. DOI: 10.1016/0378-1119(91)90434-d. View
3.
Neumann G, Watanabe T, Ito H, Watanabe S, Goto H, Gao P . Generation of influenza A viruses entirely from cloned cDNAs. Proc Natl Acad Sci U S A. 1999; 96(16):9345-50. PMC: 17785. DOI: 10.1073/pnas.96.16.9345. View
4.
Quinlivan M, Zamarin D, Garcia-Sastre A, Cullinane A, Chambers T, Palese P . Attenuation of equine influenza viruses through truncations of the NS1 protein. J Virol. 2005; 79(13):8431-9. PMC: 1143746. DOI: 10.1128/JVI.79.13.8431-8439.2005. View
5.
Fodor E, Devenish L, Engelhardt O, Palese P, Brownlee G, Garcia-Sastre A . Rescue of influenza A virus from recombinant DNA. J Virol. 1999; 73(11):9679-82. PMC: 113010. DOI: 10.1128/JVI.73.11.9679-9682.1999. View