Effect of Parathyrin on the Transport Properties of Isolated Renal Brush-border Vesicles

Overview

Journal Biochem J

Specialty Biochemistry

Date 1978 Apr 15

PMID 207266

Citations 20

Authors

C Evers

H Murer

R KINNE

Affiliations

Soon will be listed here.

Abstract

The transport properties of brush-border membrane vesicles isolated by a calcium-precipitation method from the renal cortex of normal and parathyrin (parathyroid hormone)-treated rats were studied by a rapid-filtration technique. Parathyrin elicited a dose-dependent decrease in the Na+-dependent phosphate uptake by the brush-border membrane vesicles, but the uptake of D-glucose, Na+ and mannitol was not affected. A maximum inhibition of 30% was observed after the application of 30 U.S.P. units intramuscularly 1 h before the animals were killed. Intravenous infusion of dibutyryl cyclic AMP (0.5-1.5 MG) also decreased the phosphate uptake by the brush-border vesicles. Both dibutyryl cyclic AMP and parathyrin were ineffective when added in vitro to brush-border membrane vesicles isolated from normal rats. These data suggest that parathyrin exerts its action on the phosphate reabsorption in the renal proximal tubule by affecting the Na+/phosphate co-transport system in the brush-border membrane. The effects of parathyrin on Na+ and glucose transport, however, seem to be due to alterations to the driving forces for transport and not to the brush-border transport systems.

Citing Articles

Exome sequencing identifies a novel homozygous mutation in the phosphate transporter SLC34A1 in hypophosphatemia and nephrocalcinosis.

Rajagopal A, Braslavsky D, Lu J, Kleppe S, Clement F, Cassinelli H J Clin Endocrinol Metab. 2014; 99(11):E2451-6.

PMID: 25050900 PMC: 4223446. DOI: 10.1210/jc.2014-1517.

A dibasic motif involved in parathyroid hormone-induced down-regulation of the type IIa NaPi cotransporter.

Hernando N, Biber J, Murer H Proc Natl Acad Sci U S A. 2000; 97(23):12896-901.

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Chloride uptake by brush border membrane vesicles isolated from rabbit renal cortex. Coupling to proton gradients and K+ diffusion potentials.

Warnock D, Yee V J Clin Invest. 1981; 67(1):103-15.

PMID: 7451645 PMC: 371577. DOI: 10.1172/JCI110002.

The influence of pH on phosphate transport into rat renal brush border membrane vesicles.

Burckhardt G, Stern H, Murer H Pflugers Arch. 1981; 390(2):191-7.

PMID: 7195568 DOI: 10.1007/BF00590206.

Membrane transport in the proximal tubule and thick ascending limb of Henle's loop: mechanisms and their alterations.

Murer H, Greger R Klin Wochenschr. 1982; 60(18):1103-13.

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