Guanosine Quadruplexes in Solution: a Small-angle X-ray Scattering Analysis of Temperature Effects on Self-assembling of Deoxyguanosine Monophosphate

Overview

Journal J Nucleic Acids

Publisher Wiley

Date 2010 Aug 21

PMID 20725625

Citations 4

Authors

P Mariani

F Spinozzi

F Federiconi

M G Ortore

H Amenitsch

L Spindler

I Drevensek-Olenik

Affiliations

Soon will be listed here.

Abstract

We investigated quadruplex formation in aqueous solutions of 2'-deoxyriboguanosine 5'-monophosphate, d(pG), which takes place in the absence of the covalent axial backbone. A series of in-solution small angle X-ray scattering experiments on d(pG) have been performed as a function of temperature in the absence of excess salt, at a concentration just above the critical one at which self-assembling occurs. A global fit approach has been used to derive composition and size distribution of the scattering particles as a function of temperature. The obtained results give thermodynamical justification for the observed phase-behavior, indicating that octamer formation is essential for quadruplex elongation. Our investigation shows that d(pG) quadruplexes are very suitable to assess the potential of G-quadruplex formation and to study the self-assembling thermodynamics.

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