L-FABP T94A Decreased Fatty Acid Uptake and Altered Hepatic Triglyceride and Cholesterol Accumulation in Chang Liver Cells Stably Transfected with L-FABP

Overview

Journal Mol Cell Biochem

Publisher Springer

Specialty Biochemistry

Date 2010 Aug 20

PMID 20721681

Citations 21

Authors

Na Gao

Xia Qu

Jin Yan

Qi Huang

Hao-Yong Yuan

Dong-Sheng Ouyang

Affiliations

Soon will be listed here.

Abstract

Liver fatty acid-binding protein (L-FABP, FABP1) is a highly conserved key factor in lipid metabolism. This study was undertaken to verify whether the T94A mutation in the L-FABP gene affects fatty acid uptake and intracellular esterification into specific lipid pools. Candidate SNPs were recreated using site-directed mutagenesis and tested for physical function in stably transfected Chang liver cell lines. We found that the T94A mutant of L-FABP lowered FFA uptake but had no effect on FFA efflux. L-FABP T94A-expressing cells showed decreased triglyceride content and increased cholesterol accumulation compared to the wild-type control for cells incubated with an FFA mixture (oleate: palmitate, 2:1 ratio). In conclusion, our study provided additional indications of the functional relevance of the L-FABP T94A SNP in hepatic fatty acid and lipid metabolism in humans.

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