Toward in Vivo Chemical Imaging of Epicuticular Waxes

Overview

Journal Plant Physiol

Specialty Physiology

Date 2010 Aug 17

PMID 20709828

Citations 9

Authors

Ina Weissflog

Nadine Vogler

Denis Akimov

Andrea Dellith

Doreen Schachtschabel

Ales Svatos

Wilhelm Boland

Benjamin Dietzek

Jurgen Popp

Affiliations

Soon will be listed here.

Abstract

Epicuticular waxes, which are found on the outer surface of plant cuticles, are difficult to study in vivo. To monitor the growth, development, and structural alterations of epicuticular wax layers, coherent anti-Stokes Raman scattering (CARS) might be used. CARS, as a Raman-based technique, not only provides structural insight but also chemical information by imaging the spatial distribution of Raman-active vibrations. Here, we present a comparative study using CARS and scanning electron microscopy to characterize the structure of epicuticular waxes. The ability of CARS to provide detailed structural information on the biologically important wax layer was detailed on the examples of cherry laurel (Prunus laurocerasus), hoya (Hoya carnosa), and ceriman/Swiss cheese plant (Monstera sp. aff. deliciosa). We anticipate that the work presented will open a doorway for online monitoring of formation and alterations of epicuticular wax layers.

Citing Articles

"Wax On, Wax Off": In Vivo Imaging of Plant Physiology and Disease with Fourier Transform Infrared Reflectance Microspectroscopy.

Khambatta K, Hollings A, Sauzier G, Sanglard L, Klein A, Tobin M Adv Sci (Weinh). 2021; 8(19):e2101902.

PMID: 34338438 PMC: 8498906. DOI: 10.1002/advs.202101902.

Leaf Cuticular Transpiration Barrier Organization in Tea Tree Under Normal Growth Conditions.

Chen M, Zhang Y, Kong X, Du Z, Zhou H, Yu Z Front Plant Sci. 2021; 12:655799.

PMID: 34276719 PMC: 8278822. DOI: 10.3389/fpls.2021.655799.

Raman imaging reveals in-situ microchemistry of cuticle and epidermis of spruce needles.

Sasani N, Bock P, Felhofer M, Gierlinger N Plant Methods. 2021; 17(1):17.

PMID: 33557869 PMC: 7871409. DOI: 10.1186/s13007-021-00717-6.

Plasticity of the Cuticular Transpiration Barrier in Response to Water Shortage and Resupply in : A Role of Cuticular Waxes.

Zhang Y, Du Z, Han Y, Chen X, Kong X, Sun W Front Plant Sci. 2021; 11:600069.

PMID: 33505410 PMC: 7829210. DOI: 10.3389/fpls.2020.600069.

Structure-function analysis of the maize bulliform cell cuticle and its potential role in dehydration and leaf rolling.

Matschi S, Vasquez M, Bourgault R, Steinbach P, Chamness J, Kaczmar N Plant Direct. 2020; 4(10):e00282.

PMID: 33163853 PMC: 7598327. DOI: 10.1002/pld3.282.

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