Cleavage of Epstein-Barr Virus DNA by Restriction Endonucleases EcoRI, HindIII and BamI

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1978 Apr 1

PMID 206875

Citations 32

Authors

L Rymo

S Forsblom

Affiliations

Soon will be listed here.

Abstract

The cleavage of the DNAs of the B95-8 and P3HR-1 virus strains of Epstein-Barr virus by the restriction endonucleases EcoRI, HindIII and BamI was investigated using a new technique for quantitative evaluation of the fluorescence of ethidium stained DNA fragments separated on agarose gels. The results obtained with B95-8 DNA showed that in addition to the limited repetitions of nucleotide sequences observed in the EcoRI and HindIII cleavage patterns, the molecule contained a BamI fragment with a molecular mass of 2.0 megadaltons which was present in a total of about 11 copies and localized to a limited part of the DNA molecule. The same sequences were also present in the P3HR-1 DNA albeit in a lower molar ratio. P3HR-1 DNA yielded restriction enzyme cleavage patterns suggesting DNA sequence heterogeneity of P3HR-1 virus. No fragment was present in more than about 4 copies per molecule of P3HR-1 DNA. Comparison of the restriction enzyme cleavage patterns of P3HR-1 and B95-8 DNA revealed a high degree of structural homology emphasized by nucleic acid hybridization experiments with EBV complementary RNA synthesized in vitro.

Citing Articles

Epithelial cell retention of transcriptionally active, P3HR-1-derived heterogeneous Epstein-Barr virus DNA with concurrent loss of parental virus.

Ikuta K, Ding M, Zhang F, Sixbey J, Scott R J Virol. 2011; 85(15):7634-43.

PMID: 21593154 PMC: 3147926. DOI: 10.1128/JVI.00045-11.

Physical Map of the Channel Catfish Virus Genome: Location of Sites for Restriction Endonucleases EcoRI, HindIII, HpaI, and XbaI.

Chousterman S, Lacasa M, Sheldrick P J Virol. 1979; 31(1):73-85.

PMID: 16789182 PMC: 353423. DOI: 10.1128/JVI.31.1.73-85.1979.

The EB virus genome in Daudi Burkitt's lymphoma cells has a deletion similar to that observed in a non-transforming strain (P3HR-1) of the virus.

Jones M, Foster L, Sheedy T, Griffin B EMBO J. 1984; 3(4):813-21.

PMID: 6327290 PMC: 557432. DOI: 10.1002/j.1460-2075.1984.tb01890.x.

Two distant regions of the Epstein-Barr virus genome with sequence homologies have the same orientation and involve small tandem repeats.

Hudewentz J, Delius H, Freese U, Zimber U, Bornkamm G EMBO J. 1982; 1(1):21-6.

PMID: 6325150 PMC: 552989. DOI: 10.1002/j.1460-2075.1982.tb01118.x.

Epstein-Barr virus with heterogeneous DNA disrupts latency.

Miller G, RABSON M, Heston L J Virol. 1984; 50(1):174-82.

PMID: 6321789 PMC: 255597. DOI: 10.1128/JVI.50.1.174-182.1984.

References

Sugden B . Comparison of Epstein-Barr viral DNAs in Burkitt lymphoma biopsy cells and in cells clonally transformed in vitro. Proc Natl Acad Sci U S A. 1977; 74(10):4651-5. PMC: 432005. DOI: 10.1073/pnas.74.10.4651. View

Hayward S, Kieff E . DNA of Epstein-Barr virus. II. Comparison of the molecular weights of restriction endonuclease fragments of the DNA of Epstein-Barr virus strains and identification of end fragments of the B95-8 strain. J Virol. 1977; 23(2):421-9. PMC: 515844. DOI: 10.1128/JVI.23.2.421-429.1977. View

Miller G, Shope T, Coope D, Waters L, PAGANO J, Bornkamn G . Lymphoma in cotton-top marmosets after inoculation with Epstein-Barr virus: tumor incidence, histologic spectrum antibody responses, demonstration of viral DNA, and characterization of viruses. J Exp Med. 1977; 145(4):948-67. PMC: 2180630. DOI: 10.1084/jem.145.4.948. View

Fresen K, Merkt B, Bornkamm G, Hausen H . Heterogeneity of Epstein-Barr virus originating from P3HR-1 cells. I. Studies on EBNA induction. Int J Cancer. 1977; 19(3):317-23. DOI: 10.1002/ijc.2910190306. View

Pedrali-Noy G, Weissbach A . Evidence of a repetitive sequence in vaccinia virus DNA. J Virol. 1977; 24(1):406-7. PMC: 515942. DOI: 10.1128/JVI.24.1.406-407.1977. View

McDonell M, Simon M, Studier F . Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol. 1977; 110(1):119-46. DOI: 10.1016/s0022-2836(77)80102-2. View

Forsblom S, Rigler R, Ehrenberg M, Philipson L . Kinetic studies on the cleavage of adenovirus DNA by restriction endonuclease Eco RI. Nucleic Acids Res. 1976; 3(12):3255-69. PMC: 343172. DOI: 10.1093/nar/3.12.3255. View

Miller G, Robinson J, Heston L, Lipman M . Differences between laboratory strains of Epstein-Barr virus based on immortalization, abortive infection, and interference. Proc Natl Acad Sci U S A. 1974; 71(10):4006-10. PMC: 434316. DOI: 10.1073/pnas.71.10.4006. View

Scherberg N, Refetoff S . The preparation of carrier-free iodine isotope-substituted cytosine nucleotides. Biochim Biophys Acta. 1974; 340(4):446-51. DOI: 10.1016/0005-2787(74)90065-3. View

10.

Adams A . Concentration of Epstein-Barr virus from cell culture fluids with polyethylene glycol. J Gen Virol. 1973; 20(3):391-4. DOI: 10.1099/0022-1317-20-3-391. View

11.

Hinuma Y, Konn M, Yamaguchi J, Wudarski D, Blakeslee Jr J, GRACE Jr J . Immunofluorescence and herpes-type virus particles in the P3HR-1 Burkitt lymphoma cell line. J Virol. 1967; 1(5):1045-51. PMC: 375384. DOI: 10.1128/JVI.1.5.1045-1051.1967. View

12.

Miller G, Lipman M . Release of infectious Epstein-Barr virus by transformed marmoset leukocytes. Proc Natl Acad Sci U S A. 1973; 70(1):190-4. PMC: 433213. DOI: 10.1073/pnas.70.1.190. View

13.

Shope T, DeChairo D, Miller G . Malignant lymphoma in cottontop marmosets after inoculation with Epstein-Barr virus. Proc Natl Acad Sci U S A. 1973; 70(9):2487-91. PMC: 427039. DOI: 10.1073/pnas.70.9.2487. View

14.

Pettersson U, Mulder C, Deluis H, Sharp P . Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R-RI. Proc Natl Acad Sci U S A. 1973; 70(1):200-4. PMC: 433215. DOI: 10.1073/pnas.70.1.200. View

15.

Sugden B, Summers W, Klein G . Nucleic acid renaturation and restriction endonuclease cleavage analyses show that the DNAs of a transforming and a nontransforming strain of Epstein-Barr virus share approximately 90% of their nucleotide sequences. J Virol. 1976; 18(2):765-75. PMC: 515605. DOI: 10.1128/JVI.18.2.765-775.1976. View

16.

Lindahl T, Adams A, Bjursell G, Bornkamm G, Jehn U . Covalently closed circular duplex DNA of Epstein-Barr virus in a human lymphoid cell line. J Mol Biol. 1976; 102(3):511-30. DOI: 10.1016/0022-2836(76)90331-4. View

17.

Pritchett R, Hayward S, Kieff E . DNA of Epstein-Barr virus. I. Comparative studies of the DNA of Epstein-Barr virus from HR-1 and B95-8 cells: size, structure, and relatedness. J Virol. 1975; 15(3):556-9. PMC: 354493. DOI: 10.1128/JVI.15.3.556-559.1975. View

18.

Fresen K, Hausen H . Establishment of EBNA-expressing cell lines by infection of Epstein-Barr virus (EBV)-genome-negative human lymphoma cells with different EBV strains. Int J Cancer. 1976; 17(2):161-6. DOI: 10.1002/ijc.2910170203. View

19.

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

20.

Southern E . Long range periodicities in mouse satellite DNA. J Mol Biol. 1975; 94(1):51-69. DOI: 10.1016/0022-2836(75)90404-0. View