The Novel Responses of Ethambutol Against Mycobacterium Smegmatis Mc²155 Revealed by Proteomics Analysis

Overview

Journal Curr Microbiol

Specialty Microbiology

Date 2010 Aug 6

PMID 20686769

Citations 5

Authors

Tao Jiang

Yaoyao Zhan

Mingzhong Sun

Shuqing Liu

Shizhu Zang

Yufang Ma

Yi Xin

Affiliations

Soon will be listed here.

Abstract

Ethambutol (EMB), one of the effective anti-mycobacterial drugs, inhibits the biosynthesis of mycobacterium cell wall. To elucidate the molecular mechanism of EMB against tuberculosis (TB), Mycobacterium smegmatis mc²155 was employed as a model of mycobacterial system in this study. We compared the protein profiles on M. smegmatis mc²155 treated by EMB and untreated using fluorescence difference two-dimensional gel electrophoresis (2-D DIGE). A total of 40 differential protein spots were selected and 22 proteins were identified by HPLC-nano ESI-MS/MS analysis, including 16 over-expressed proteins and 6 under-expressed proteins. These proteins mainly affected energy metabolism, as well as synthesis and modification of macromolecules. The expressions of correspondent genes were confirmed by RT-PCR. This investigation provided some clues for searching potential drug targets.

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