Effects of Lead and Mercury on the Blood Proteome of Children

Overview

Journal J Proteome Res

Publisher American Chemical Society

Specialty Biochemistry

Date 2010 Aug 5

PMID 20681587

Citations 11

Authors

Robert E Birdsall

Michael P Kiley

Zaneer M Segu

Christopher D Palmer

Milan Madera

Brooks B Gump

James A MacKenzie

Patrick J Parsons

Yehia Mechref

Milos V Novotny

Kestutis G Bendinskas

Affiliations

Soon will be listed here.

Abstract

Heavy metal exposure in children has been associated with a variety of physiological and neurological problems. The goal of this study was to utilize proteomics to enhance the understanding of biochemical interactions responsible for the health problems related to lead and mercury exposure at concentrations well below CDC guidelines. Blood plasma and serum samples from 34 children were depleted of their most abundant proteins using antibody-based affinity columns and analyzed using two different methods, LC-MS/MS and 2-D electrophoresis coupled with MALDI-TOF/MS and tandem mass spectrometry. Apolipoprotein E demonstrated an inverse significant association with lead concentrations (average being one microgram/deciliter) as deduced from LC-MS/MS and 2-D electrophoresis and confirmed by Western blot analysis. This coincides with prior findings that Apolipoprotein E genotype moderates neurobehavioral effects in individuals exposed to lead. Fifteen other proteins were identified by LC-MS/MS as proteins of interest exhibiting expressional differences in the presence of environmental lead and mercury.

Citing Articles

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