A Comparative Study of Merkel Cell, BK and JC Polyomavirus Infections in Renal Transplant Recipients and Healthy Subjects

Overview

Journal J Clin Virol

Specialty Microbiology

Date 2010 Jul 30

PMID 20667770

Citations 32

Authors

Mohamed I Husseiny

Bishoy Anastasi

Jennifer Singer

Simon F Lacey

Affiliations

Soon will be listed here.

Abstract

Background: Merkel cell carcinoma (MCC) is a rare skin cancer associated with immunosuppression and the integration of Merkel cell polyomavirus (MCPyV) DNA into the tumor cell genome. Little is known about the natural history of MCPyV infection.

Objectives: To investigate the presence of MCPyV, BK and JC polyomaviruses in serum and urine from immunosuppressed kidney transplant patients (KTx) and a control group of normal volunteers.

Study Design: Quantitative real-time PCR (q-PCR) was used to assess MCPyV, BKV and JCV viral load in urine and serum samples collected from normal donors (Group A), prospectively enrolled KTx patients (Group B) and from KTx with documented BK reactivation and/or nephropathy (Group C).

Results: Low levels of MCPyV viruria was seen in 15% of the subjects in Group A, 30% of Group B, and was not detected in Group C. No individuals in the study developed MCPyV viremia. BK viruria was seen in 5% of Group A, 30% of Group B, and 100% of Group C. Consistent with previous reports, the mean BKV urinary load was significantly higher in immunosuppressed patients compared to non-immunosuppressed controls and also higher in urine compared to serum samples.

Conclusions: Like BKV and JCV, MCPyV is likely a common infection in adult humans. Low level shedding of MCPyV in urine was similar in immunosuppressed organ transplant recipients to non-immunosuppressed subjects. However, MCPyV was not detected and JCV was infrequent in samples from KTx patients with clinical BKV reactivation.

Citing Articles

Neutralizing Antibodies Targeting BK Polyomavirus: Clinical Importance and Therapeutic Potential for Kidney Transplant Recipients.

Helle F, Aubry A, Morel V, Descamps V, Demey B, Brochot E J Am Soc Nephrol. 2024; 35(10):1425-1433.

PMID: 39352862 PMC: 11452134. DOI: 10.1681/ASN.0000000000000457.

Longitudinal study of human polyomaviruses viruria in kidney transplant recipients.

Dolci M, Colico C, Ambrogi F, Favi E, Signorini L, Perego M Clin Exp Med. 2024; 24(1):3.

PMID: 38231278 PMC: 10794280. DOI: 10.1007/s10238-023-01290-z.

Human polyomaviruses JCPyV and MCPyV in urothelial cell carcinoma: a single institution experience.

Klufah F, Mobaraki G, Shi S, Marcelissen T, Alharbi R, Mobarki M Front Oncol. 2024; 13:1251244.

PMID: 38192628 PMC: 10773619. DOI: 10.3389/fonc.2023.1251244.

Analysis of Several Common APOBEC-type Mutations in Bladder Tumors Suggests Links to Viral Infection.

Rao N, Starrett G, Piaskowski M, Butler K, Golubeva Y, Yan W Cancer Prev Res (Phila). 2023; 16(10):561-570.

PMID: 37477495 PMC: 10592262. DOI: 10.1158/1940-6207.CAPR-23-0112.

Merkel Cell Polyomavirus: Infection, Genome, Transcripts and Its Role in Development of Merkel Cell Carcinoma.

Houben R, Celikdemir B, Kervarrec T, Schrama D Cancers (Basel). 2023; 15(2).

PMID: 36672392 PMC: 9857234. DOI: 10.3390/cancers15020444.

References

Nickeleit V, Hirsch H, Binet I, Gudat F, Prince O, Dalquen P . Polyomavirus infection of renal allograft recipients: from latent infection to manifest disease. J Am Soc Nephrol. 1999; 10(5):1080-9. DOI: 10.1681/ASN.V1051080. View

Loyo M, Guerrero-Preston R, Brait M, Hoque M, Chuang A, Kim M . Quantitative detection of Merkel cell virus in human tissues and possible mode of transmission. Int J Cancer. 2009; 126(12):2991-6. PMC: 2856748. DOI: 10.1002/ijc.24737. View

Duncavage E, Zehnbauer B, Pfeifer J . Prevalence of Merkel cell polyomavirus in Merkel cell carcinoma. Mod Pathol. 2009; 22(4):516-21. DOI: 10.1038/modpathol.2009.3. View

Penn I, First M . Merkel's cell carcinoma in organ recipients: report of 41 cases. Transplantation. 1999; 68(11):1717-21. DOI: 10.1097/00007890-199912150-00015. View

Kazory A, Ducloux D, Chalopin J, Angonin R, Fontaniere B, Moret H . The first case of JC virus allograft nephropathy. Transplantation. 2004; 76(11):1653-5. DOI: 10.1097/01.TP.0000090749.42791.14. View

Nickeleit V, Klimkait T, Binet I, Dalquen P, Del Zenero V, Thiel G . Testing for polyomavirus type BK DNA in plasma to identify renal-allograft recipients with viral nephropathy. N Engl J Med. 2000; 342(18):1309-15. DOI: 10.1056/NEJM200005043421802. View

Milstone A, Vilchez R, Geiger X, Fogo A, Butel J, Dummer S . Polyomavirus simian virus 40 infection associated with nephropathy in a lung-transplant recipient. Transplantation. 2004; 77(7):1019-24. DOI: 10.1097/01.tp.0000119156.52197.ca. View

Feng H, Shuda M, Chang Y, Moore P . Clonal integration of a polyomavirus in human Merkel cell carcinoma. Science. 2008; 319(5866):1096-100. PMC: 2740911. DOI: 10.1126/science.1152586. View

Hodgson N . Merkel cell carcinoma: changing incidence trends. J Surg Oncol. 2004; 89(1):1-4. DOI: 10.1002/jso.20167. View

10.

Hirsch H, Knowles W, Dickenmann M, Passweg J, Klimkait T, Mihatsch M . Prospective study of polyomavirus type BK replication and nephropathy in renal-transplant recipients. N Engl J Med. 2002; 347(7):488-96. DOI: 10.1056/NEJMoa020439. View

11.

Goh S, Lindau C, Tiveljung-Lindell A, Allander T . Merkel cell polyomavirus in respiratory tract secretions. Emerg Infect Dis. 2009; 15(3):489-91. PMC: 2681127. DOI: 10.3201/eid1503.081206. View

12.

Sharp C, Norja P, Anthony I, Bell J, Simmonds P . Reactivation and mutation of newly discovered WU, KI, and Merkel cell carcinoma polyomaviruses in immunosuppressed individuals. J Infect Dis. 2008; 199(3):398-404. DOI: 10.1086/596062. View

13.

Binggeli S, Egli A, Schaub S, Binet I, Mayr M, Steiger J . Polyomavirus BK-specific cellular immune response to VP1 and large T-antigen in kidney transplant recipients. Am J Transplant. 2007; 7(5):1131-9. DOI: 10.1111/j.1600-6143.2007.01754.x. View

14.

Swann M, Yoon J . Merkel cell carcinoma. Semin Oncol. 2007; 34(1):51-6. DOI: 10.1053/j.seminoncol.2006.10.021. View

15.

Kassem A, Schopflin A, Diaz C, Weyers W, Stickeler E, Werner M . Frequent detection of Merkel cell polyomavirus in human Merkel cell carcinomas and identification of a unique deletion in the VP1 gene. Cancer Res. 2008; 68(13):5009-13. DOI: 10.1158/0008-5472.CAN-08-0949. View

16.

Ridd K, Yu S, Bastian B . The presence of polyomavirus in non-melanoma skin cancer in organ transplant recipients is rare. J Invest Dermatol. 2008; 129(1):250-2. PMC: 2837873. DOI: 10.1038/jid.2008.215. View

17.

Foulongne V, Kluger N, Dereure O, Brieu N, Guillot B, Segondy M . Merkel cell polyomavirus and Merkel cell carcinoma, France. Emerg Infect Dis. 2008; 14(9):1491-3. PMC: 2603124. DOI: 10.3201/eid1409.080651. View

18.

Hirsch H, Brennan D, Drachenberg C, Ginevri F, Gordon J, Limaye A . Polyomavirus-associated nephropathy in renal transplantation: interdisciplinary analyses and recommendations. Transplantation. 2005; 79(10):1277-86. DOI: 10.1097/01.tp.0000156165.83160.09. View

19.

Moens U, Johannessen M . Human polyomaviruses and cancer: expanding repertoire. J Dtsch Dermatol Ges. 2008; 6(9):704-8. DOI: 10.1111/j.1610-0387.2008.06810.x. View

20.

Becker J, Houben R, Ugurel S, Trefzer U, Pfohler C, Schrama D . MC polyomavirus is frequently present in Merkel cell carcinoma of European patients. J Invest Dermatol. 2008; 129(1):248-50. DOI: 10.1038/jid.2008.198. View