Mammalian Nucleotide Excision Repair Proteins and Interstrand Crosslink Repair

Overview

Journal Environ Mol Mutagen

Specialties Environmental Health
Molecular Biology

Date 2010 Jul 27

PMID 20658645

Citations 59

Authors

Richard D Wood

Affiliations

Soon will be listed here.

Abstract

Although various schemes for interstrand crosslink (ICL) repair incorporate DNA recombination, replication, and double-strand break intermediate steps, action of the nucleotide excision repair (NER) system or some variation of it is a common feature of most models. In the bacterium Escherichia coli, the NER enzyme UvrABC can incise on either side of an ICL to unhook the crosslink, and can proceed via a subsequent recombination step. The relevance of NER to ICL repair in mammalian cells has been challenged. Of all NER mutants, it is clear that ERCC1 and XPF-defective cells show the most pronounced sensitivities to ICL-inducing agents, and defects in ICL repair. However, there is good evidence that cells defective in NER proteins including XPA and XPG are also more sensitive than normal to ICL-inducing agents. These results are summarized here, together with evidence for defective crosslink removal in NER-defective cells. Studies of incision at sites of ICL by cell extracts and purified proteins have been done, but these studies are not all consistent with one another and further research is required.

Citing Articles

XPD stalled on cross-linked DNA provides insight into damage verification.

Kuper J, Hove T, Maidl S, Neitz H, Sauer F, Kempf M Nat Struct Mol Biol. 2024; 31(10):1580-1588.

PMID: 38806694 PMC: 11479942. DOI: 10.1038/s41594-024-01323-5.

Replication-Independent ICL Repair: From Chemotherapy to Cell Homeostasis.

Ahmed A, Kato N, Gautier J J Mol Biol. 2024; 436(13):168618.

PMID: 38763228 PMC: 11227339. DOI: 10.1016/j.jmb.2024.168618.

When DNA-damage responses meet innate and adaptive immunity.

Tong J, Song J, Zhang W, Zhai J, Guan Q, Wang H Cell Mol Life Sci. 2024; 81(1):185.

PMID: 38630271 PMC: 11023972. DOI: 10.1007/s00018-024-05214-2.

A Brief Review on Chemoresistance; Targeting Cancer Stem Cells as an Alternative Approach.

Toledo B, Gonzalez-Titos A, Hernandez-Camarero P, Peran M Int J Mol Sci. 2023; 24(5).

PMID: 36901917 PMC: 10003376. DOI: 10.3390/ijms24054487.

The multiple de novo copy number variant (MdnCNV) phenomenon presents with peri-zygotic DNA mutational signatures and multilocus pathogenic variation.

Du H, Jolly A, Grochowski C, Yuan B, Dawood M, Jhangiani S Genome Med. 2022; 14(1):122.

PMID: 36303224 PMC: 9609164. DOI: 10.1186/s13073-022-01123-w.

References

Clingen P, Arlett C, Hartley J, Parris C . Chemosensitivity of primary human fibroblasts with defective unhooking of DNA interstrand cross-links. Exp Cell Res. 2006; 313(4):753-60. DOI: 10.1016/j.yexcr.2006.11.007. View

Araujo S, Tirode F, Coin F, Pospiech H, Syvaoja J, Stucki M . Nucleotide excision repair of DNA with recombinant human proteins: definition of the minimal set of factors, active forms of TFIIH, and modulation by CAK. Genes Dev. 2000; 14(3):349-59. PMC: 316364. View

Sinden R, Cole R . Topography and kinetics of genetic recombination in Escherichia coli treated with psoralen and light. Proc Natl Acad Sci U S A. 1978; 75(5):2373-7. PMC: 392555. DOI: 10.1073/pnas.75.5.2373. View

Thoma B, Wakasugi M, Christensen J, Reddy M, Vasquez K . Human XPC-hHR23B interacts with XPA-RPA in the recognition of triplex-directed psoralen DNA interstrand crosslinks. Nucleic Acids Res. 2005; 33(9):2993-3001. PMC: 1140082. DOI: 10.1093/nar/gki610. View

Min J, Pavletich N . Recognition of DNA damage by the Rad4 nucleotide excision repair protein. Nature. 2007; 449(7162):570-5. DOI: 10.1038/nature06155. View

Andersson B, Sadeghi T, Siciliano M, LEGERSKI R, Murray D . Nucleotide excision repair genes as determinants of cellular sensitivity to cyclophosphamide analogs. Cancer Chemother Pharmacol. 1996; 38(5):406-16. DOI: 10.1007/s002800050504. View

Sinden R, Cole R . Repair of cross-linked DNA and survival of Escherichia coli treated with psoralen and light: effects of mutations influencing genetic recombination and DNA metabolism. J Bacteriol. 1978; 136(2):538-47. PMC: 218577. DOI: 10.1128/jb.136.2.538-547.1978. View

Cole R . Repair of DNA containing interstrand crosslinks in Escherichia coli: sequential excision and recombination. Proc Natl Acad Sci U S A. 1973; 70(4):1064-8. PMC: 433426. DOI: 10.1073/pnas.70.4.1064. View

Shen X, Do H, Li Y, Chung W, Tomasz M, de Winter J . Recruitment of fanconi anemia and breast cancer proteins to DNA damage sites is differentially governed by replication. Mol Cell. 2009; 35(5):716-23. PMC: 2758766. DOI: 10.1016/j.molcel.2009.06.034. View

10.

Sladek F, Munn M, RUPP W, HOWARD-FLANDERS P . In vitro repair of psoralen-DNA cross-links by RecA, UvrABC, and the 5'-exonuclease of DNA polymerase I. J Biol Chem. 1989; 264(12):6755-65. View

11.

Ben-Yehoyada M, Wang L, Kozekov I, Rizzo C, Gottesman M, Gautier J . Checkpoint signaling from a single DNA interstrand crosslink. Mol Cell. 2009; 35(5):704-15. PMC: 2756577. DOI: 10.1016/j.molcel.2009.08.014. View

12.

Gordienko I, RUPP W . A specific 3' exonuclease activity of UvrABC. EMBO J. 1998; 17(2):626-33. PMC: 1170412. DOI: 10.1093/emboj/17.2.626. View

13.

Evans E, Moggs J, Hwang J, Egly J, Wood R . Mechanism of open complex and dual incision formation by human nucleotide excision repair factors. EMBO J. 1997; 16(21):6559-73. PMC: 1170260. DOI: 10.1093/emboj/16.21.6559. View

14.

Yokoi M, Masutani C, Maekawa T, Sugasawa K, Ohkuma Y, Hanaoka F . The xeroderma pigmentosum group C protein complex XPC-HR23B plays an important role in the recruitment of transcription factor IIH to damaged DNA. J Biol Chem. 2000; 275(13):9870-5. DOI: 10.1074/jbc.275.13.9870. View

15.

Camenisch U, Trautlein D, Clement F, Fei J, Leitenstorfer A, Ferrando-May E . Two-stage dynamic DNA quality check by xeroderma pigmentosum group C protein. EMBO J. 2009; 28(16):2387-99. PMC: 2735174. DOI: 10.1038/emboj.2009.187. View

16.

Sladek F, Melian A, HOWARD-FLANDERS P . Incision by UvrABC excinuclease is a step in the path to mutagenesis by psoralen crosslinks in Escherichia coli. Proc Natl Acad Sci U S A. 1989; 86(11):3982-6. PMC: 287372. DOI: 10.1073/pnas.86.11.3982. View

17.

Paques F, Haber J . Multiple pathways of recombination induced by double-strand breaks in Saccharomyces cerevisiae. Microbiol Mol Biol Rev. 1999; 63(2):349-404. PMC: 98970. DOI: 10.1128/MMBR.63.2.349-404.1999. View

18.

Mu D, Bessho T, Nechev L, Chen D, Harris T, Hearst J . DNA interstrand cross-links induce futile repair synthesis in mammalian cell extracts. Mol Cell Biol. 2000; 20(7):2446-54. PMC: 85433. DOI: 10.1128/MCB.20.7.2446-2454.2000. View

19.

Camenisch U, Dip R, Briand Schumacher S, Schuler B, Naegeli H . Recognition of helical kinks by xeroderma pigmentosum group A protein triggers DNA excision repair. Nat Struct Mol Biol. 2006; 13(3):278-84. DOI: 10.1038/nsmb1061. View

20.

Staresincic L, Fagbemi A, Enzlin J, Gourdin A, Wijgers N, Dunand-Sauthier I . Coordination of dual incision and repair synthesis in human nucleotide excision repair. EMBO J. 2009; 28(8):1111-20. PMC: 2683701. DOI: 10.1038/emboj.2009.49. View