Article: Molecular cloning, genomic structure and expression analysis of major histocompatibility complex class Iα gene of half-smooth tongue sole (Cynoglossus semilaevis)

Major histocompatibility complex (MHC) has a central role in the adaptive immune system by presenting foreign peptide to the T-cell receptor. The full length of MHC class Iα cDNA was cloned from half-smooth tongue sole by homology cloning and rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR), genomic organization and expression of MHC Iα were examined to study the function of MHC gene in fish. The domain structure feature and antigen-binding motifs of other teleost and mammals MHC are conserved in the half-smooth tongue sole MHC Iα gene. The deduced amino acid sequence of half-smooth tongue sole MHC Iα (GenBank accession no. FJ372720) had 12.1-61.8% identity with those of human and other fish. Eight exons and seven introns were identified in MHC Iα gene. Real-time quantitative PCR demonstrated that MHC Iα gene was ubiquitously expressed in normal tissues, while that in Vibrio anguillarum infected fish was significantly increased in intestines and decreased in spleen and liver from 24 to 72 h after infection, followed by a recovery to normal level after 96 h.

Citing Articles

The draft genome of the large yellow croaker reveals well-developed innate immunity.

Wu C, Zhang D, Kan M, Lv Z, Zhu A, Su Y Nat Commun. 2014; 5:5227.

PMID: 25407894 PMC: 4263168. DOI: 10.1038/ncomms6227.

Gene cloning and expression analysis of IRF1 in half-smooth tongue sole (Cynoglossus semilaevis).

Lu Y, Wang Q, Liu Y, Shao C, Chen S, Sha Z Mol Biol Rep. 2014; 41(6):4093-101.

PMID: 24584575 DOI: 10.1007/s11033-014-3279-2.

References

1.

Salter R, Norment A, Chen B, Clayberger C, Krensky A, Littman D . Polymorphism in the alpha 3 domain of HLA-A molecules affects binding to CD8. Nature. 1989; 338(6213):345-7. DOI: 10.1038/338345a0. View

2.

Kaufman J, Salomonsen J, Flajnik M . Evolutionary conservation of MHC class I and class II molecules--different yet the same. Semin Immunol. 1994; 6(6):411-24. DOI: 10.1006/smim.1994.1050. View

3.

Lukacs M, Harstad H, Grimholt U, Beetz-Sargent M, Cooper G, Reid L . Genomic organization of duplicated major histocompatibility complex class I regions in Atlantic salmon (Salmo salar). BMC Genomics. 2007; 8:251. PMC: 1971071. DOI: 10.1186/1471-2164-8-251. View

4.

Stet R, de Vries B, Mudde K, Hermsen T, van Heerwaarden J, Shum B . Unique haplotypes of co-segregating major histocompatibility class II A and class II B alleles in Atlantic salmon (Salmo salar) give rise to diverse class II genotypes. Immunogenetics. 2002; 54(5):320-31. DOI: 10.1007/s00251-002-0477-1. View

5.

Kruiswijk C, Hermsen T, Westphal A, Savelkoul H, Stet R . A novel functional class I lineage in zebrafish (Danio rerio), carp (Cyprinus carpio), and large barbus (Barbus intermedius) showing an unusual conservation of the peptide binding domains. J Immunol. 2002; 169(4):1936-47. DOI: 10.4049/jimmunol.169.4.1936. View

6.

Hashimoto K, Nakanishi T, Kurosawa Y . Isolation of carp genes encoding major histocompatibility complex antigens. Proc Natl Acad Sci U S A. 1990; 87(17):6863-7. PMC: 54638. DOI: 10.1073/pnas.87.17.6863. View

7.

Antao A, Wilson M, Wang J, Bengten E, Miller N, Clem L . Genomic organization and differential expression of channel catfish MHC class I genes. Dev Comp Immunol. 2001; 25(7):579-95. DOI: 10.1016/s0145-305x(01)00017-9. View

8.

Lunney J, Ho C, Wysocki M, Smith D . Molecular genetics of the swine major histocompatibility complex, the SLA complex. Dev Comp Immunol. 2008; 33(3):362-74. DOI: 10.1016/j.dci.2008.07.002. View

9.

Meagher S, Potts W . A microsatellite-based MHC genotyping system for house mice (Mus domesticus). Hereditas. 1997; 127(1-2):75-82. DOI: 10.1111/j.1601-5223.1997.00075.x. View

10.

Xu T, Chen S, Ji X, Tian Y . MHC polymorphism and disease resistance to Vibrio anguillarum in 12 selective Japanese flounder (Paralichthys olivaceus) families. Fish Shellfish Immunol. 2008; 25(3):213-21. DOI: 10.1016/j.fsi.2008.05.007. View

11.

Xu T, Chen S, Ji X, Sha Z . Molecular cloning, genomic structure, polymorphism and expression analysis of major histocompatibility complex class IIA and IIB genes of half-smooth tongue sole (Cynoglossus semilaevis). Fish Shellfish Immunol. 2009; 27(2):192-201. DOI: 10.1016/j.fsi.2009.04.009. View

Molecular Cloning, Genomic Structure and Expression Analysis of Major Histocompatibility Complex Class Iα Gene of Half-smooth Tongue Sole (Cynoglossus Semilaevis)