Rhizoma Coptidis Inhibits LPS-induced MCP-1/CCL2 Production in Murine Macrophages Via an AP-1 and NFkappaB-dependent Pathway

Overview

Journal Mediators Inflamm

Publisher Wiley

Specialties Biochemistry
Pathology

Date 2010 Jul 24

PMID 20652055

Citations 35

Authors

Andrew Remppis

Florian Bea

Henry Johannes Greten

Annette Buttler

Hongjie Wang

Qianxing Zhou

Michael R Preusch

Ronny Enk

Robert Ehehalt

Hugo Katus

Erwin Blessing

Affiliations

Soon will be listed here.

Abstract

Introduction: The Chinese extract Rhizoma coptidis is well known for its anti-inflammatory, antioxidative, antiviral, and antimicrobial activity. The exact mechanisms of action are not fully understood.

Methods: We examined the effect of the extract and its main compound, berberine, on LPS-induced inflammatory activity in a murine macrophage cell line. RAW 264.7 cells were stimulated with LPS and incubated with either Rhizoma coptidis extract or berberine. Activation of AP-1 and NFkappaB was analyzed in nuclear extracts, secretion of MCP-1/CCL2 was measured in supernatants.

Results: Incubation with Rhizoma coptidis and berberine strongly inhibited LPS-induced monocyte chemoattractant protein (MCP)-1 production in RAW cells. Activation of the transcription factors AP-1 and NFkappaB was inhibited by Rhizoma coptidis in a dose- and time-dependent fashion.

Conclusions: Rhizoma coptidis extract inhibits LPS-induced MCP-1/CCL2 production in vitro via an AP-1 and NFkappaB-dependent pathway. Anti-inflammatory action of the extract is mediated mainly by its alkaloid compound berberine.

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