Pituitary Adenylate Cyclase-activating Polypeptide is Protective Against Oxidative Stress in Human Retinal Pigment Epithelial Cells

Overview

Journal J Mol Neurosci

Specialties Molecular Biology
Neurology

Date 2010 Jul 21

PMID 20645022

Citations 11

Authors

Laszlo Mester

Krisztina Kovacs

Boglarka Racz

Izabella Solti

Tamas Atlasz

Krisztina Szabadfi

Andrea Tamas

Dora Reglodi

Affiliations

Soon will be listed here.

Abstract

Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide with potent neurotrophic and neuroprotective effects. We have previously shown that PACAP protects against several types of retinal injuries in vivo, including retinal ischemia, glutamate-induced excitotoxicity, UV A-induced lesion, and diabetic retinopathy. We have also shown that PACAP activates antiapoptotic pathways and inhibits proapoptotic signaling in retinal lesions in vivo. PACAP receptors have been identified on the retinal pigment epithelial cells and PACAP has been shown to inhibit interleukin secretion from pigment epithelial cells. It is not known, however, whether PACAP is protective in these cells. Human retinal pigment epithelial cells (ARPE-19 cell line) were exposed to in vitro oxidative stress by hydrogen peroxide. Cell survival was decreased in cells exposed to oxidative stress, which could be significantly and dose-dependently attenuated by 10 pM-1 μM PACAP treatment, as shown by MTT viability test. The protective effect of PACAP could be blocked by the receptor antagonist PACAP6-38. In addition, flow cytometry and JC-1 assay revealed that oxidative stress-induced apoptosis in retinal pigment epithelial cells could be decreased by PACAP treatment. In summary, these results show, for the first time, that PACAP is antiapoptotic in the retinal pigment epithelial cells.

Citing Articles

Effects of Pituitary Adenylate Cyclase Activating Polypeptide on Cell Death.

Horvath G, Reglodi D, Fabian E, Opper B Int J Mol Sci. 2022; 23(9).

PMID: 35563353 PMC: 9100246. DOI: 10.3390/ijms23094953.

PACAP and NAP: Effect of Two Functionally Related Peptides in Diabetic Retinopathy.

DAmico A, Maugeri G, Musumeci G, Reglodi D, DAgata V J Mol Neurosci. 2021; 71(8):1525-1535.

PMID: 33403597 DOI: 10.1007/s12031-020-01769-4.

Protective Effects of PACAP in Peripheral Organs.

Toth D, Szabo E, Tamas A, Juhasz T, Horvath G, Fabian E Front Endocrinol (Lausanne). 2020; 11:377.

PMID: 32765418 PMC: 7381171. DOI: 10.3389/fendo.2020.00377.

PACAP Attenuates Optic Nerve Crush-Induced Retinal Ganglion Cell Apoptosis Via Activation of the CREB-Bcl-2 Pathway.

Ye D, Shi Y, Xu Y, Huang J J Mol Neurosci. 2019; 68(3):475-484.

PMID: 30993644 DOI: 10.1007/s12031-019-01309-9.

The synovial fluid neuropeptide PACAP may act as a protective factor during disease progression of primary knee osteoarthritis and is increased following hyaluronic acid injection.

Sun Z, Wu S, Liang C, Zhao C, Sun B Innate Immun. 2019; 25(4):255-264.

PMID: 30935267 PMC: 6830887. DOI: 10.1177/1753425919839125.

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