Theory of Site-specific DNA-protein Interactions in the Presence of Conformational Fluctuations of DNA Binding Domains

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 2010 Jul 21

PMID 20643052

Citations 18

Authors

R Murugan

Affiliations

Soon will be listed here.

Abstract

We develop a theory that explains how the thermally driven conformational fluctuations in the DNA binding domains (DBDs) of the DNA binding proteins (DBPs) are effectively coupled to the one-dimensional searching dynamics of DBPs for their cognate sites on DNA. We show that the rate gammaopt, associated with the flipping of conformational states of DBDs beyond which the maximum search efficiency of DBPs is achieved, varies with the one-dimensional sliding length L as gammaopt proportional, L(-2) and with the number of roadblock protein molecules present on the same DNA m as gammaopt proportional, m2. The required free energy barrier ERTO associated with this flipping transition seems to be varying with L as ERTO proportional, variant ln L2. When the barrier height associated with the conformational flipping of DBDs is comparable with that of the thermal free energy, then the possible value of L under in vivo conditions seems to be L<or=70 bps.

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