» Articles » PMID: 206295

Stochastic ESR Analysis of Rat Liver and Hepatoma Mitochondrial Lipids

Overview
Specialty Biophysics
Date 1978 Apr 13
PMID 206295
Affiliations
Soon will be listed here.
Abstract

A commonly used model for the interaction of the motional narrowing of ESR lines is shown to be qualitatively misleading. An analysis of lipid extracts of mitochondrial preparations labeled with 12-nitroxide stearic acid produced linear plots of the logarithm of the correlation time versus the reciprocal of the absolute temperature when analyzed with stochastic computer simulations. However, when the data were analyzed with isotropic Lorentzian line shape approximations, nonlinear plots were obtained.

References
1.
Jost P, Griffith O . The molecular reorganization of lipid bilayers by osmium tetroxide. A spin-label study of orientation and restricted y-axis anisotropic motion in model membrane systems. Arch Biochem Biophys. 1973; 159(1):70-81. DOI: 10.1016/0003-9861(73)90430-x. View

2.
Raison J, McMurchie E . Two temperature-induced changes in mitochondrial membranes detected by spin labelling and enzyme kinetics. Biochim Biophys Acta. 1974; 363(2):135-40. DOI: 10.1016/0005-2736(74)90053-4. View

3.
Vorbeck M, Oswald T, Martin A . Preparation of subcellular fractions from rat liver: comparison of the Polytron with the Dounce homogenizer. Prep Biochem. 1976; 6(5):387-402. DOI: 10.1080/00327487608061626. View

4.
Mehlhorn R, SNIPES W, Keith A . Spin label motion in fatty acids. Biophys J. 1973; 13(11):1223-31. PMC: 1484379. DOI: 10.1016/S0006-3495(73)86057-6. View

5.
Jost P, Libertini L, Hebert V, Griffith O . Lipid spin labels in lecithin multilayers. A study of motion along fatty acid chains. J Mol Biol. 1971; 59(1):77-98. DOI: 10.1016/0022-2836(71)90414-1. View