Cyclic AMP-dependent Histone-specific Nucleoplasmic Protein Kinase from Rat Liver

Overview

Journal Biochem J

Specialty Biochemistry

Date 1978 Apr 1

PMID 206265

Citations 2

Authors

J R Neumann

A R OMeara

R L HERRMANN

Affiliations

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Abstract

A nucleoplasmic histone kinase activity was isolated from livers of adult rats and purified 39-fold compared with whole nuclei by ultracentrifugation of the nuclear extract and Sephadex G-200 gel filtration in the presence of cyclic AMP. Analysis by polyacrylamide-gel electrophoresis as well as by gel filtration indicates a mol.wt. of approx. 60,000 for the catalytic subunit and 130000-150000 for the cyclic AMP-binding activity. The purified enzyme displays a 20-fold greater preference for histone fractions 1 and 2b than for any non-histone substrate, including alpha-casein. Endogenous protein in the preparation is not appreciably phosphorylated. The unfractioned enzyme is stimulated significantly by cyclic GMP, cyclic IMP and dibutyryl cyclic AMP as well as by cyclic AMP. The catalytic reaction requires Mg2+ (Km 1.9mM) and ATP (Km 15.4 micron). Half-maximal activity of the enzyme is observed with histone 2b at 12micron and histone 1 at a higher substrate concentration. The pH optima are 6.1 and 6.5 with histones 2b and 1 respectively. This nuclear protein kinase appears to be distinct from other nuclear enzymes that have been reported, on the basis of histone specificity, univalent-salt-sensitivity, pH optima and nuclear location. However, the enzyme possesses many properties similar to those of the cytoplasmic kinases, including cyclic AMP-dependence, Mg2+ and ATP affinities and pH optima. It differs from cytoplasmic protein kinase type I, the major form in the liver, with respect to bivalent-cation effects and response to the heat-stable protein kinase inhibitor protein isolated from ox heart.

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References

Costa E, Kurosawa A, Guidotti A . Activation and nuclear translocation of protein kinase during transsynaptic induction of tyrosine 3-monooxygenase. Proc Natl Acad Sci U S A. 1976; 73(4):1058-62. PMC: 430199. DOI: 10.1073/pnas.73.4.1058. View

Farago A, ROMHANYI T, Antoni F, Takats A, Fabian F . The phosphorylated site of calf thymus F2b histone by the cyclic AMP-dependent protein kinase. Nature. 1975; 254(5495):88. DOI: 10.1038/254088a0. View

Jungmann R, Lee S, DeAngelo A . Translocation of cytoplasmic protein kinase and cyclic adenosine monophosphate-binding protein to intracellular acceptor sites. Adv Cyclic Nucleotide Res. 1975; 5:281-306. View

Gill G, Kanstein C . Guanosine 3',5'-monophosphate receptor protein: separation from adenosine 3',5'-monophosphate receptor protein. Biochem Biophys Res Commun. 1975; 63(4):1113-22. DOI: 10.1016/0006-291x(75)90684-1. View

Corbin J, Keely S, Park C . The distribution and dissociation of cyclic adenosine 3':5'-monophosphate-dependent protein kinases in adipose, cardiac, and other tissues. J Biol Chem. 1975; 250(1):218-25. View

Johnson E, Hadden J, Inoue A, Allfrey V . DNA binding by cyclic adenosine 3',5'-monophosphate dependent protein kinase from calf thymus nuclei. Biochemistry. 1975; 14(17):3873-84. DOI: 10.1021/bi00688a022. View

Castagna M, Palmer W, Walsh D . Nuclear protein-kinase activity in perfused rat liver stimulated with dibutyryl-adenosine cyclic 3':5'-monophosphate. Eur J Biochem. 1975; 55(1):193-9. DOI: 10.1111/j.1432-1033.1975.tb02151.x. View

Dahmus M . Stimulation of ascites tumor RNA polymerase II by protein kinase. Biochemistry. 1976; 15(9):1821-9. DOI: 10.1021/bi00654a006. View

Nesterova M, Sashchenko L, Vasiliev V, Severin E . A cyclic adenosine 3',5'-monophosphate-dependent histone kinase from pig brain. Purification and some properties of the enzyme. Biochim Biophys Acta. 1975; 377(2):271-81. DOI: 10.1016/0005-2744(75)90309-5. View

10.

Umansky S, Zotova R, Kovalev Y . Comparison of some properties of chromatin non-histone proteins and nuclear sap proteins. Eur J Biochem. 1976; 65(2):503-12. DOI: 10.1111/j.1432-1033.1976.tb10366.x. View

11.

Weintraub H, WORCEL A, Alberts B . A model for chromatin based upon two symmetrically paired half-nucleosomes. Cell. 1976; 9(3):409-17. DOI: 10.1016/0092-8674(76)90085-4. View

12.

Sherod D, Johnson G, Balhorn R, Jackson V, Chalkley R, Granner D . The phosphorylation region of lysine-rich histone in dividing cells. Biochim Biophys Acta. 1975; 381(2):337-47. DOI: 10.1016/0304-4165(75)90239-1. View

13.

Weintraub H, Palter K, Van Lente F . Histones H2a, H2b, H3, and H4 form a tetrameric complex in solutions of high salt. Cell. 1975; 6(1):85-110. DOI: 10.1016/0092-8674(75)90077-x. View

14.

Keller R, Chandra T, Schrader W, OMalley B . Protein kinases of the chick oviduct: a study of the cytoplasmic and nuclear enzymes. Biochemistry. 1976; 15(9):1958-67. DOI: 10.1021/bi00654a025. View

15.

Goldberg N, ODea R, Haddox M . Cyclic GMP. Adv Cyclic Nucleotide Res. 1973; 3:155-223. View

16.

Meisler M, LANGAN T . Characterization of a phosphatase specific for phosphorylated histones and protamine. J Biol Chem. 1969; 244(18):4961-8. View

17.

Miyamoto E, Kuo J, Greengard P . Cyclic nucleotide-dependent protein kinases. 3. Purification and properties of adenosine 3',5'-monophosphate-dependent protein kinase from bovine brain. J Biol Chem. 1969; 244(23):6395-402. View

18.

Pastan I, Perlman R . Cyclic adenosine monophosphate in bacteria. Science. 1970; 169(3943):339-44. DOI: 10.1126/science.169.3943.339. View

19.

GILMAN A . A protein binding assay for adenosine 3':5'-cyclic monophosphate. Proc Natl Acad Sci U S A. 1970; 67(1):305-12. PMC: 283204. DOI: 10.1073/pnas.67.1.305. View

20.

Brostrom M, Reimann E, Walsh D, KREBS E . A cyclic 3',5'-amp-stimulated protein kinase from cardiac muscle. Adv Enzyme Regul. 1970; 8:191-203. DOI: 10.1016/0065-2571(70)90017-8. View