Ecm29 Fulfils Quality Control Functions in Proteasome Assembly

Overview

Journal Mol Cell

Publisher Cell Press

Specialty Cell Biology

Date 2010 Jul 13

PMID 20620957

Citations 40

Authors

Andrea Lehmann

Agathe Niewienda

Katharina Jechow

Katharina Janek

Cordula Enenkel

Affiliations

Soon will be listed here.

Abstract

The proteasome, the central protease of eukaryotic cells, is composed of one core particle (CP) and one or two adjacent regulatory particles (RP), which contain multiple subunits. Several proteasome-dedicated chaperones govern the assembly of CP and RP, respectively. We sought for proteins that regulate final steps of RP-CP assembly in yeast and found Ecm29, a conserved HEAT-like repeat protein. Here, we show that Ecm29 controls the integrity of RP-CP assemblies. Ecm29 recognizes RP-CP species in which CP maturation is stalled due to the lack of distinct beta subunits. Reconstitution assays revealed that Ecm29 functions as scaffold protein during the remodeling of incompletely matured RP-CP assemblies into regular enzymes. Upon the completion of CP maturation, Ecm29 is degraded and RP-CP is dissociated.

Citing Articles

The Structural Role of RPN10 in the 26S Proteasome and an RPN2-Binding Residue on RPN13 Are Functionally Important in Arabidopsis.

Lin S, Lin Y, Usharani R, Radjacommare R, Fu H Int J Mol Sci. 2024; 25(21).

PMID: 39519207 PMC: 11546751. DOI: 10.3390/ijms252111650.

A novel endoplasmic reticulum adaptation is critical for the long-lived Caenorhabditis elegans rpn-10 proteasomal mutant.

Chinchankar M, Taylor W, Ko S, Apple E, Rodriguez K, Chen L Biochim Biophys Acta Gene Regul Mech. 2023; 1866(3):194957.

PMID: 37355092 PMC: 10528105. DOI: 10.1016/j.bbagrm.2023.194957.

Proteasome-Associated Proteins, PA200 and ECPAS, Are Essential for Murine Spermatogenesis.

Sato B, Kim J, Morohoshi K, Kang W, Miyado K, Tsuruta F Biomolecules. 2023; 13(4).

PMID: 37189334 PMC: 10135743. DOI: 10.3390/biom13040586.

Assembly chaperone Nas6 selectively destabilizes 26S proteasomes with defective regulatory particle-core particle interfaces.

Warnock J, Jobin G, Kumar S, Tomko Jr R J Biol Chem. 2023; 299(2):102894.

PMID: 36634850 PMC: 9943895. DOI: 10.1016/j.jbc.2023.102894.

Adhesion dynamics in the neocortex determine the start of migration and the post-migratory orientation of neurons.

Epifanova E, Salina V, Lajko D, Textoris-Taube K, Naumann T, Bormuth O Sci Adv. 2021; 7(27).

PMID: 34215578 PMC: 11060048. DOI: 10.1126/sciadv.abf1973.