SMN, Gemin2 and Gemin3 Associate with Beta-actin MRNA in the Cytoplasm of Neuronal Cells in Vitro

Overview

Journal J Mol Biol

Publisher Elsevier

Specialties Microbiology
Molecular Biology

Date 2010 Jul 13

PMID 20620147

Citations 23

Authors

Adrian G Todd

Robert Morse

Debra J Shaw

Samuel McGinley

Howard Stebbings

Philip J Young

Affiliations

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Abstract

Childhood spinal muscular atrophy is caused by a reduced expression of the survival motor neuron (SMN) protein. SMN has been implicated in the axonal transport of beta-actin mRNA in both primary and transformed neuronal cell lines, and loss of this function could account, at least in part, for spinal muscular atrophy onset and pathological specificity. Here we have utilised a targeted screen to identify mRNA associated with SMN, Gemin2 and Gemin3 in the cytoplasm of a human neuroblastoma cell line, SHSY5Y. Importantly, we have provided the first direct evidence that beta-actin mRNA is present in SMN cytoplasmic complexes in SHSY5Y cells.

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