Identification and Characterization of Genes Associated with Tapping Panel Dryness from Hevea Brasiliensis Latex Using Suppression Subtractive Hybridization

Overview

Journal BMC Plant Biol

Publisher Biomed Central

Specialty Biology

Date 2010 Jul 13

PMID 20618931

Citations 23

Authors

Dejun Li

Zhi Deng

Chunliu Chen

Zhihui Xia

Min Wu

Peng He

Shoucai Chen

Affiliations

Soon will be listed here.

Abstract

Background: Tapping panel dryness (TPD) is one of the most serious threats to natural rubber production. Although a great deal of effort has been made to study TPD in rubber tree, the molecular mechanisms underlying TPD remain poorly understood. Identification and systematical analyses of the genes associated with TPD are the prerequisites for elucidating the molecular mechanisms involved in TPD. The present study is undertaken to generate information about the genes related to TPD in rubber tree.

Results: To identify the genes related to TPD in rubber tree, forward and reverse cDNA libraries from the latex of healthy and TPD trees were constructed using suppression subtractive hybridization (SSH) method. Among the 1106 clones obtained from the two cDNA libraries, 822 clones showed differential expression in two libraries by reverse Northern blot analyses. Sequence analyses indicated that the 822 clones represented 237 unique genes; and most of them have not been reported to be associated with TPD in rubber tree. The expression patterns of 20 differentially expressed genes were further investigated to validate the SSH data by reverse transcription PCR (RT-PCR) and real-time PCR analysis. According to the Gene Ontology convention, 237 unique genes were classified into 10 functional groups, such as stress/defense response, protein metabolism, transcription and post-transcription, rubber biosynthesis, etc. Among the genes with known function, the genes preferentially expressed were associated with stress/defense response in the reverse library, whereas metabolism and energy in the forward one.

Conclusions: The genes associated with TPD were identified by SSH method in this research. Systematic analyses of the genes related to TPD suggest that the production and scavenging of reactive oxygen species (ROS), ubiquitin proteasome pathway, programmed cell death and rubber biosynthesis might play important roles in TPD. Therefore, our results not only enrich information about the genes related to TPD, but also provide new insights into understanding the TPD process in rubber tree.

Citing Articles

The Role of Plant Latex in Virus Biology.

Merchan-Gaitan J, Mendes J, Nunes L, Buss D, Rodrigues S, Fernandes P Viruses. 2024; 16(1).

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Downregulation of HbFPS1 affects rubber biosynthesis of Hevea brasiliensis suffering from tapping panel dryness.

Nie Z, Kang G, Yan D, Qin H, Yang L, Zeng R Plant J. 2022; 113(3):504-520.

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Transcriptome analysis of Pará rubber tree (H. brasiliensis) seedlings under ethylene stimulation.

Nakano Y, Mitsuda N, Ide K, Mori T, Mira F, Rosmalawati S BMC Plant Biol. 2021; 21(1):420.

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Identification and Characterization of Glycoproteins and Their Responsive Patterns upon Ethylene Stimulation in the Rubber Latex.

Yu L, Yuan B, Wang L, Sun Y, Ding G, Souleymane O Int J Mol Sci. 2020; 21(15).

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Plant Defense by Latex: Ecological Genetics of Inducibility in the Milkweeds and a General Review of Mechanisms, Evolution, and Implications for Agriculture.

Agrawal A, Hastings A J Chem Ecol. 2019; 45(11-12):1004-1018.

PMID: 31755020 DOI: 10.1007/s10886-019-01119-8.

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