Dual Regulation of Mu Opioid Receptors in SK-N-SH Neuroblastoma Cells by Morphine and Interleukin-1β: Evidence for Opioid-immune Crosstalk

Overview

Journal J Neuroimmunol

Specialty Neurology

Date 2010 Jul 10

PMID 20615556

Citations 11

Authors

Shekher Mohan

Randall L Davis

Udaya DeSilva

Craig W Stevens

Affiliations

Soon will be listed here.

Abstract

Treatment of SK-N-SH cells with morphine and interleukin-1beta (IL-1β) produced dual regulation of the mRNA for the human mu opioid receptor (MOR) protein. Morphine produced a decrease in the MOR mRNA while IL-1β increased it, as assessed by real-time quantitative PCR. These data were consistent with immunocytochemical studies of treated and untreated cells. Morphine-mediated down-regulation of MOR was blocked by naltrexone and IL-1β-induced up-regulation of MOR was blocked by interleukin-1 receptor type 1 antagonist. Immune-opioid crosstalk was examined by IL-1β and morphine co-treatment. These data are the first to show dual regulation of MOR in neuroblastoma cells.

Citing Articles

Morphine-mediated release of astrocyte-derived extracellular vesicle miR-23a induces loss of pericyte coverage at the blood-brain barrier: Implications for neuroinflammation.

Liao K, Niu F, Hu G, Buch S Front Cell Dev Biol. 2022; 10:984375.

PMID: 36478740 PMC: 9720401. DOI: 10.3389/fcell.2022.984375.

Molecular mechanisms of opioid tolerance: From opioid receptors to inflammatory mediators (Review).

Zhou J, Ma R, Jin Y, Fang J, Du J, Shao X Exp Ther Med. 2021; 22(3):1004.

PMID: 34345286 PMC: 8311239. DOI: 10.3892/etm.2021.10437.

Upregulation of μ-Opioid Receptor in the Rat Spinal Cord Contributes to the α2-Adrenoceptor Agonist Dexmedetomidine-Induced Attenuation of Chronic Morphine Tolerance in Cancer Pain.

Zhang P, Bu J, Wu X, Deng L, Chi M, Ma C J Pain Res. 2020; 13:2617-2627.

PMID: 33116804 PMC: 7573317. DOI: 10.2147/JPR.S274225.

The standardized Withania somnifera Dunal root extract alters basal and morphine-induced opioid receptor gene expression changes in neuroblastoma cells.

Caputi F, Acquas E, Kasture S, Ruiu S, Candeletti S, Romualdi P BMC Complement Altern Med. 2018; 18(1):9.

PMID: 29316911 PMC: 5761194. DOI: 10.1186/s12906-017-2065-9.

OPRM1 Methylation Contributes to Opioid Tolerance in Cancer Patients.

Viet C, Dang D, Aouizerat B, Miaskowski C, Ye Y, Viet D J Pain. 2017; 18(9):1046-1059.

PMID: 28456745 PMC: 5918413. DOI: 10.1016/j.jpain.2017.04.001.

References

Xu Y, CARR L . Binding of Sp1/Sp3 to the proximal promoter of the hMOR gene is enhanced by DAMGO. Gene. 2001; 274(1-2):119-28. DOI: 10.1016/s0378-1119(01)00624-2. View

Borner C, Kraus J, Schroder H, Ammer H, Hollt V . Transcriptional regulation of the human mu-opioid receptor gene by interleukin-6. Mol Pharmacol. 2004; 66(6):1719-26. DOI: 10.1124/mol.104.003806. View

ONeill L . Interleukin-1 signal transduction. Int J Clin Lab Res. 1995; 25(4):169-77. DOI: 10.1007/BF02592694. View

ONeill L, Kaltschmidt C . NF-kappa B: a crucial transcription factor for glial and neuronal cell function. Trends Neurosci. 1997; 20(6):252-8. DOI: 10.1016/s0166-2236(96)01035-1. View

Hutchinson M, Coats B, Lewis S, Zhang Y, Sprunger D, Rezvani N . Proinflammatory cytokines oppose opioid-induced acute and chronic analgesia. Brain Behav Immun. 2008; 22(8):1178-89. PMC: 2783238. DOI: 10.1016/j.bbi.2008.05.004. View

Wan L, Lin C, Lin Y, Sheu J, Chen B, Liao C . Type I IFN induced IL1-Ra expression in hepatocytes is mediated by activating STAT6 through the formation of STAT2: STAT6 heterodimer. J Cell Mol Med. 2008; 12(3):876-88. PMC: 4401122. DOI: 10.1111/j.1582-4934.2008.00143.x. View

Hibi M, Hirano T . Signal transduction through cytokine receptors. Int Rev Immunol. 1999; 17(1-4):75-102. DOI: 10.3109/08830189809084488. View

Law P, Wong Y, Loh H . Molecular mechanisms and regulation of opioid receptor signaling. Annu Rev Pharmacol Toxicol. 2000; 40:389-430. DOI: 10.1146/annurev.pharmtox.40.1.389. View

Kraus J, Borner C, Lendeckel U, Hollt V . Interferon-gamma down-regulates transcription of the mu-opioid receptor gene in neuronal and immune cells. J Neuroimmunol. 2006; 181(1-2):13-8. DOI: 10.1016/j.jneuroim.2006.07.012. View

10.

ONeill L . Signal transduction pathways activated by the IL-1 receptor/toll-like receptor superfamily. Curr Top Microbiol Immunol. 2002; 270:47-61. View

11.

Kraus J, Borner C, Giannini E, Hickfang K, Braun H, Mayer P . Regulation of mu-opioid receptor gene transcription by interleukin-4 and influence of an allelic variation within a STAT6 transcription factor binding site. J Biol Chem. 2001; 276(47):43901-8. DOI: 10.1074/jbc.M107543200. View

12.

Vidal E, Patel N, Wu G, Fiala M, Chang S . Interleukin-1 induces the expression of mu opioid receptors in endothelial cells. Immunopharmacology. 1998; 38(3):261-6. DOI: 10.1016/s0162-3109(97)00085-4. View

13.

Bare L, Mansson E, Yang D . Expression of two variants of the human mu opioid receptor mRNA in SK-N-SH cells and human brain. FEBS Lett. 1994; 354(2):213-6. DOI: 10.1016/0014-5793(94)01129-x. View

14.

Allan S, Tyrrell P, Rothwell N . Interleukin-1 and neuronal injury. Nat Rev Immunol. 2005; 5(8):629-40. DOI: 10.1038/nri1664. View

15.

Yu X, Mao X, Blake A, Li W, Chang S . Morphine and endomorphins differentially regulate micro-opioid receptor mRNA in SHSY-5Y human neuroblastoma cells. J Pharmacol Exp Ther. 2003; 306(2):447-54. DOI: 10.1124/jpet.103.048694. View

16.

Horner K, Zadina J . Internalization and down-regulation of mu opioid receptors by endomorphins and morphine in SH-SY5Y human neuroblastoma cells. Brain Res. 2004; 1028(2):121-32. DOI: 10.1016/j.brainres.2004.07.055. View

17.

Harrison L, Kastin A, Zadina J . Opiate tolerance and dependence: receptors, G-proteins, and antiopiates. Peptides. 1998; 19(9):1603-30. DOI: 10.1016/s0196-9781(98)00126-0. View

18.

Baumhaker Y, Gafni M, Keren O, Sarne Y . Selective and interactive down-regulation of mu- and delta-opioid receptors in human neuroblastoma SK-N-SH cells. Mol Pharmacol. 1993; 44(2):461-7. View

19.

Borner C, Hollt V, Kraus J . Involvement of activator protein-1 in transcriptional regulation of the human mu-opioid receptor gene. Mol Pharmacol. 2002; 61(4):800-5. DOI: 10.1124/mol.61.4.800. View

20.

Sarne Y, Rubovitch V, Fields A, Gafni M . Dissociation between the inhibitory and stimulatory effects of opioid peptides on cAMP formation in SK-N-SH neuroblastoma cells. Biochem Biophys Res Commun. 1998; 246(1):128-31. DOI: 10.1006/bbrc.1998.8582. View